Serum-Free Media

show/hide all

What kind of protein is in the media?

Contains less then 30µg protein/ml such as albumin, fibronectin and fetuin serve a variety of functions that include adsorbing toxic compounds, providing protection against shear forces in bioreactors, creating a matrix for cellular attachment to surfaces, and acting as a carrier for lipids and other growth factors.

Category: Serum-Free Media

Are there any type of growth factors or other stimulatory molecules added?

No, there aren’t any growth factors or other stimulatory molecules added

Category: Serum-Free Media

Is it tested for endotoxin?

Yes, it is tested for endotoxin.

Category: Serum-Free Media

Is there any albumin in the serum, either bovine or human?

Yes, as above mentioned Hu Albumin (HSA).

Category: Serum-Free Media

How many days can the media support cell growth?

The media was primarily designed for short term cultures(e.g. running short assays)

Category: Serum-Free Media

What are the reagent quantities to develop 20 plates?

58 vials of PBMC (10 million cells per vial each), 500 ml of wash media (1 x 100 ml of CTL-Wash™supplement), and 1000 ml of test media (2 x 500 mlCTL-Test™ media)

Category: Serum-Free Media

Can the CryoABC freezing media mixture be stored at +4, if yes for how long without loosing its activity?

Yes, it can be stored at 4°C for up to a week.

Category: Serum-Free Media

How long can the supplemented Test medium with L-Glutamine be stored?

For up to a week at 4°C.

Category: Serum-Free Media