S6 Ultimate M2

The spot counting accuracy of the ImmunoSpot® Single-Color ELISPOT Enzymatic Software Suite is based on rigorous experimental validation. This software implements “a single click of a button” counting algorithm based on 25 years of leading scientific expertise in ELISPOT. In “expert” mode, this software enables scientists to modify any counting parameter and to perform high content analysis based on spot sizes and morphologies, for example, to establish cell productivity and affinity distributions of antigen-specific B cells. Built in user authentication, audit trail and data security support transparent ELISPOT counting in a regulated environment.
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Two color ELISPOT analysis can be performed using precipitating substrates that have distinct color spectra with visible/white light illumination (without involving fluorescence).

The ImmunoSpot® Double Color Enzymatic Software Suite permits precise counting of single-color spots for either analyte, as well as double color spots produced by cells that co-express both analytes (J. Immunol. 2000, 164:1862). CTL offers a wide selection of double color enzymatic ELISPOT kits enabling such dual color enzymatic ELISPOT analysis.

The accurate evaluation of multicolour B-cell and T cell FluoroSpot assays, as well as BioSpot assays, each require different image analysis approaches for identifying single or multiple analyte-secreting cells.

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Immune monitoring efforts frequently involve measuring serum antibodies' efficacy in neutralizing viruses, bacteria or toxins. ImmunoSpot® readers fully support such high throughput measurements, including colony counting. Each type of colony or viral plaque has unique morphologies requiring specialized image analysis solutions. The BioSpot™ Software Suite has been established to meet this challenge. The BioSpot™ Software can distinguish and count independently objects/colonies of up to 12 different visible colors with white light illumination.

CFR Part 11 compliant QC and audit trails support transparent colony counting in a regulated environment.

CTL’s philosophy is to offer instruments that support several functions commonly performed in immune monitoring laboratories. Enabled by the BioSpot™ Software Suite, ImmunoSpot® Readers are rendered high-end colony counters.

To view the full spectrum of assays that can be analysed with the BioSpot™ Software, follow this link please.

CTL’s LDA Cell Counting Suite enables high-throughput live/dead/apoptotic (LDA) cell counting with suitable ImmunoSpot® Readers. CFR Part 11 compliant QC and audit trails support cell counting in a regulated environment.
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The Target cell Visualization Assay (TVA™) Suite is a high-throughput cell counting application that is designed to measure the lysis of fluorescence-labelled target cells in a 96-well or Terrasaki plate format. The TVA-Suite enables streamlined work flow from planning the experiment to automated generation of graphs with audit trails supporting work in an academic or regulated environment.
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Enabled by CTL’s ODense™ Software Suite, ImmunoSpot® Instruments are capable of reading ELISA results in addition to nucleic acid and protein quantification. The work flow is streamlined from planning the plate lay-out to automated generation of the graphs, and the statistical analysis of the results. CFR Part 11 compliant QC and audit trails support work in a regulated environment.

CTL’s philosophy is to offer instruments that support several functions commonly performed in immune monitoring laboratories, saving our customers the expense of having to purchase and maintain a dedicated instrument for each, all included in the cost of a single device.

Zoom lenses enable image capture at maximal resolution using different magnifications/well formats. The zoom range of lenses used in high-end ImmunoSpot® Readers extends to the microscopic scale enabling high resolution analysis of single cells. See examples of cells imaged on ImmunoSpot® Readers.

The SpotStat® Software Suite was developed for the statistical analysis of ELISPOT/Fluorospot data in immune monitoring, vaccine testing, and other fields of immunological or medical research.  SpotStat® implements a number of powerful parametric and non-parametric statistical methods for easy, streamlined analysis of data generated by CTL’s ImmunoSpot® platform. Learn More

The Image Annotator™ facilitates image analysis-oriented exchange between scientists themselves, or scientists and CTL's expert support team. Regions of interst on a well image are marked and annotated. This information along with the raw image and the counting parameters are bundled together to be passed on for discussion or consultation.

High throughput analysis is streamlined by the SpotMap™ Software Suite. In the assay-planning phase, plate layouts are created, and barcodes are generated for each plate. SpotMap™ links the spot counts for groups of repetitive wells (e.g. triplicates) to the donor sample IDs and antigens present in those wells, and passes on this information for automated statistical analysis and subsequent export to any standard database. With SpotMap™ complex assays can be evaluated “at a click of a button”.

The Reagent Tracker™ Suite provides audit trails for the accurate implementation of ELISPOT/FlouroSpot assays, revealing pipetting errors made (https://doi.org/10.3390/cells7010003). Biologically neutral dyes are used to colour different antigens and the Reagent Tracker™ Software checks whether in each assigned well the right antigen/colour has indeed been plated (revealing qualitative pipetting errors), and whether the antigen pipetting volume was correct (revealing quantitative pipetting errors). The Reagent Tracker™ Suite generates automatic printable reports and is also ideally suited for proficiency testing of lab personnel, and for training of novices in the lab.

CTL has been committed to elevating ELISPOT/FluoroSpot assays so they can meet regulatory requirements for transparency and audit trails. The Reagent Tracker™ Suite is one of the important steps in that direction.

The ImmunoQualify™ Tool permits to verify main functions of ImmunoSpot® Readers and assures accurate performance before each use, or periodically. At the “press of a button”, a Reference Plate is scanned, and the newly established counts are compared to the factory-established reference counts for that plate, generating a pass/fail report.

Starting June 1st 2023, CTL is providing a barcoded ELISPOT reference plate with every Analyzer delivery. The ExpressQualify™ tool automatically scans and counts the reference plate to verify that the Analyzer is still performing within factory specifications. To meet formal regulatory requirements and documentation, please consult our full-version ImmunoQualify™ package.

About 1,000 of our ImmunoSpot® Readers operate in a regulated environment, having satisfied in house and third-party audits, including audits of regulatory agencies. An optional ImmunoCompliance™ package is available. We offer IQ and OQ for our regulated customers. Learn more.

ImmunoSpot® Readers are frequently run in BSL 3 and even BSL-4 environments. These instruments’ skin and interior has been designed to withstand and facilitate disinfection.

Cytokine arrays can be visualized with precipitating substrates. An example, printed into a well of a 96 well plate, is shown on here. CTL’s ImmArray Software permits high throughput analysis of such assay results.

Cytokine arrays can be visualized via fluorescent detection reagents. An example, printed into a well of a 96 well plate, is shown on here. CTL’s ImmArray Software permits high throughput analysis of such assay results.

In addition to being able to scan microtiter plates of various well formats, and haemocytometers, ImmunoSpot® Readers can also scan histologic slides, enabling histologic analysis.