Video Library

Introduction

A Brief History of Studying Immunity and the Role T Cell ImmunoSpot^® Plays in It

The Unique Features of B Cell ELISPOT

Multi-color ImmunoSpot^® Analysis

ELISPOT Assays in 384 Well Format

CD8 Effector vs. Memory T Cells and CD4 Subpopulations

Measuring the Affinity of the Antigen-Specific T Cell Repertoire

Monitoring Antigen-Specific CD8 Cells and the (Lack of Usefulness) of Epitope Predictions

"Size/Density Gating" for T Cell ImmunoSpot^® Analysis

Defining the Cut-Off for Positive vs. Negative T Cell ImmunoSpot^® Counts

Positive Controls for Detection of CD4 and CD8 Memory Cells

Why We Do Not Recommend "Resting" of Cryopreserved PBMC

FAQ: How We Know Whether Antigen-Induced Cytokine Production Is T Cell Derived?

FAQ: Is There Value in Supplementing Antigen Presenting Cells?

FAQ: Specifics of the T Cell Assay – Cell Numbers, Kinetics, etc.

ImmunoSpot^® Enables In-Depth Assessment of Memory B Cells

B Cell Assays Can Be Readily Validated

Memory B Cells Reflect More Reliably on B Cell Immunity Than Serum Antibodies Do

The NK Target Cell Killing Vitalization Assay, NK-TVA

Novel Applications of ImmunoSpot^® Readers for Fluorescent Microscopy and Single Cell Analysis

BioSpot^®: Using ImmunoSpot^® Readers Towards High Throughput Neutralization Measurement

Prewetting of PVDF Plate

Replacing Underdrain

Create a Humidified Chamber

Thawing Cryopreserved PBMC

Plating Cells and Tapping Plate

FluorSpot: Final Wash and Vacuum Fold

Rinse Plate and Remove Underdrain

Diluting the Blood

Isolating PBMC by Density Gradient Centrifugation: Method 1

Isolating PBMC by Density Gradient Centrifugation: Method 2

Isolating PBMC by Density Gradient Centrifugation: Method 3

Collect Mononuclear Cells

Resuspending the PBMC Pellet