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ImmunoSpot® Kits & Components

ImmunoSpot® Kit Categories

ImmunoSpot® Kit Categories

T Cell Kits

T Cell Kits contain capture and detection antibodies, enzymatic or fluorescent detection reagents, diluent buffers and PVDF membrane plates.

Human NHP Mouse Rat Pig

B Cell Kits

B Cell Kits contain capture and detection antibodies, enzymatic or fluorescent detection reagents, diluent buffers and PVDF membrane plates.

Human Ferret Mouse Pig

Cell Counting Kits & Components

Live/dead counting of PBMC selectively stained for live, dead and apoptotic cells.

Cell Counting Kits & Components

Live/dead counting of PBMC selectively stained for live, dead and apoptotic cells.

Why choose our ELISPOT and FluoroSpot kits

Standardization and Reduced Variability

All of CTL’s kits have been optimized with serum-free media to reduce variability and eliminate the need for blocking steps.

Maximized Sensitivity for T and B Cell Detection

The ImmunoSpot® test kits are specifically optimized to achieve maximal sensitivity. This is crucial for detecting and analyzing the secretory footprint of individual T and B cells.

Detailed Subpopulation Analysis of T Cells

CTL’s multiplexed ELISPOT/FluoroSpot T cell kits are suited for optimal detection and analysis of both CD4+ and CD8+ subpopulations. Learn More.

All Inclusive Serum-free Media

CTL’s serum-free media platform includes freezing, thawing, testing media and has been formulated for low background and high antigen-specific signal performance in T cell ImmunoSpot® assays. Learn More.

PBMC

CTL offers PBMC with pre-defined properties for assay development. Such samples serve as invaluable positive controls and reference samples for assay validation. Learn More.

Comprehensive Support and Resources

Need help?

CTL’s Kit Service Team offers assay development support as well as scanning and analysis services. Contact Us.

NOT SEEING A PARTICULAR DETECTION SYSTEM OF INTEREST?

CTL will gladly develop one to fit your specific assay needs.

Featured Resources

Antibody Levels Poorly Reflect on the Frequency of Memory B Cells Generated following SARS-CoV-2, Seasonal Influenza, or EBV Infection

Antibody levels weakly correlate with memory B cells for SARS-CoV-2, influenza, and Epstein–Barr virus. Direct monitoring of memory B cells is necessary for accurate immune assessment.

Learn more

CERI, CEFX, and CPI: Largely Improved Positive Controls for Testing Antigen-Specific T Cell Function in PBMC Compared to CEF

Reliable T cell immune monitoring requires assessing PBMC functional fitness using universal controls for CD8+ and CD4+ T cells and APCs, addressing potential variables.

Learn more

ImmunoSpot® Video Library

Explore our extensive video library showcasing the exceptional capabilities of the ImmunoSpot® platform.

Learn more

Frequently Asked Questions

  • What comes in the kit?

    • CTL T cell enzymatic kits come with the plates, diluents, capture antibody(s) (if not pre-coated), detection antibody(s), enzyme-conjugated tertiary antibody(s), substrates(s) and serum-free culture medium, CTL-Test.
    • CTL B cell enzymatic kits come with plates, diluents, capture antibody(s), detection antibody(s), enzyme-conjugated tertiary antibody(s), substrates(s) and B-poly-S.
    • CTL T cell fluorescent kits come with the plates, diluents, capture antibody(s) (if not pre-coated), detection antibody(s), fluorescently conjugated tertiary antibody(s) and serum-free culture medium, CTL-Test.
    • CTL B cell fluorescent kits come with plates, diluents, capture antibody(s), detection antibody(s), fluorescently conjugated tertiary antibody(s) and B-Poly-S.

  • What are the optimal storage conditions for the ELISPOT and FluoroSpot kits?

    All kits should be stored at 4-8°C. Non-pre-coated plates can be stored at room temperature and B-Poly-S should be removed from B-cell kits, preferably aliquoted for single use and stored at -20°C.

  • What is the expiration date for kits?

    All kits come with a minimum of a 6-month expiration date.