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Noémi Becza, Lingling Yao, Paul V. Lehmann, and Greg A. Kirchenbaum.

Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot® Analysis of Antigen-Specific Memory B Cells

Greg A. Kirchenbaum, Graham Pawelec, and Paul V. Lehmann.

The Importance of Monitoring Antigen-Specific Memory B Cells, and How ImmunoSpot® Assays Are Suitable for This Task

Paul V. Lehmann, Alexey Y. Karulin, Noémi Becza, Lingling Yao, Zhigang Liu, Jack Chepke, Andrea Maul-Pavicic, Carla Wolf, Sebastian Köppert, Alexis V. Valente, Anton V. Gorbachev, Magdalena Tary-Lehmann and Greg A. Kirchenbaum.

Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot® assays

Paul V. Lehmann, Zhigang Liu, Noémi Becza, Alexis V. Valente, Junbo Wang, and Greg A. Kirchenbaum.

Monitoring memory B cells by next generation ImmunoSpot® provides insights into humoral immunity that measurements of circulating antibodies do not reveal

Lingling Yao, Noémi Becza, Andrea Maul-Pavicic, Jack Chepke, Greg A. Kirchenbaum, and Paul V. Lehmann.

Four-color ImmunoSpot® assays requiring only 1-3 mL of blood permit precise frequency measurements of antigen-specific B cells secreting immunoglobulins of all four classes and subclasses

Noémi Becza, Zhigang Liu, Jack Chepke, Xing-Huang Gao, Paul V. Lehmann, and Greg A. Kirchenbaum.

Assessing the affinity spectrum of the antigen-specific B cell repertoire via ImmunoSpot®

Alexey Y. Karulin, Melinda Katona, Zoltán Megyesi, Greg A. Kirchenbaum and Paul V. Lehmann.

Artificial intelligence-based counting algorithm enables accurate and detailed analysis of the broad spectrum of spot morphologies observed in antigen-specific B cell ELISPOT and FluoroSpot assays

Alexander A. Lehmann, Diana R. Roen, Zoltan Megyesi, and Paul V. Lehmann.

Reagent Tracker™ platform verifies and provides audit trails for the error-free implementation of T cell ImmunoSpot® assays

Paul V. Lehmann, Diana R. Roen, Alexander A. Lehmann.

Unbiassed, high-troughput identification of T cell epitopes by ELISPOT

Carla Wolf, Sebastian Köppert, Noémi Becza, Stefanie Kuerten, Greg A. Kirchenbaum, Paul V. Lehmann.

Antibody levels poorly reflect on the frequency of memory B cells generated following SARS-CoV-2, seasonal influenza, or EBV infection.

Joseph Eiden, Gilad Gordon, Carlos Fierro, Renee Herber, Roger Aitchison, Robert Belshe, Harry Greenberg, Daniel Hoft, Yasuko Hatta, Michael J Moser, Magdalena Tary-Lehmann, Yoshihiro Kawaoka, Gabriele Neumann, Paul Radspinner, Pamuk Bilsel.

Safety and Immunogenicity of M2-Deficient, Single Replication, Live Influenza Vaccine (M2SR) in Adults.

Sharon E Frey, Jack T Stapleton, Zuhair K Ballas, Wendy L Rasmussen, Thomas M Kaufman, Tammy P Blevins, Travis L Jensen, D Huw Davies, Magdalena Tary-Lehmann, Paul Chaplin, Heather Hill, Johannes B Goll, DMID 09-0002 MVA Vaccine Study Group.

Human Antibody Responses Following Vaccinia Immunization Using Protein Microarrays and Correlation with Cell-Mediated Immunity and Antibody-Dependent Cellular Cytotoxicity Responses.

Sebastian Köppert, Carla Wolf, Noémi Becza, Giuseppe A Sautto, Fridolin Franke, Stefanie Kuerten, Ted M Ross, Paul V Lehmann, Greg A Kirchenbaum.

Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays

Alexander A. Lehmann, Greg A Kirchenbaum, Ting Zhang, Pedro A Reche, Paul V. Lehmann.

Deconvoluting the T cell response to SARS-CoV-2: specificity versus chance- and cognate cross-reactivity.

Alexander A. Lehmann, Pedro A. Reche, Ting Zhang, Maneewan Suwansaard, and Paul. V. Lehmann.

CERI, CEF-X, and CPI: largely improved positive controls for testing antigen-specific T cell function in PBMC compared to CEF.

Alexander A. Lehmann, Ting Zhang, Pedro A Reche, Paul V. Lehmann.

Discordance between the predicted vs. the actually recognized CD8+ T cell epitopes of HCMV pp65 antigen and aleatory epitope dominance

Anna Przybyla, Alexander A. Lehmann, Ting Zhang, Jacek Mackiewicz, Łukasz Galus, Greg A. Kirchenbaum, Andrzej Mackiewicz, Paul V. Lehmann.

Functional T cell reactivity to melanocyte antigens is lost during development of malignant melanoma, but is restored with successful immunotherapy.

Alexander Lehmann and Paul V. Lehmann.

Aleatory epitope recognition prevails in human T cell responses?

Fridolin Franke, Greg A. Kirchenbaum, Stefanie Kuerten and Paul V. Lehmann.

IL-21 in conjunction with anti-CD40 and IL-4 constitutes a potent polyclonal B cell stimulator for monitoring antigen-specific memory B cells.

Paul V. Lehmann, Maneewan Suwansaard, Ting Zhang, Diana R. Roen, Greg A. Kirchenbaum, Alexey Y. Karulin, Alexander Lehmann, and Pedro A. Reche.

Comprehensive evaluation of the expressed CD8+ T cell epitope space using high-throughput epitope mapping.

Kirchenbaum GA, Hanson J, Roen DR, Lehmann PV.

Detection of Antigen-Specific T Cell Lineages and Effector Functions Based on Secretory Signature.

Fredrik Terlutter, Tobias M. Nowacki, Richard Caspell, Ruliang Li, Krisztian Horvath, Charlotte von Gall, Stefanie Kurten, Srividya Sundararaman, and Paul V. Lehmann.

Direct detection of T and B memory lymphocytes reveals HCMV exposure that serum antibodies fail to identify.

Fecher P., Caspell R., Naeem, V., Karulin A.Y., Kuerten S.and P.V. Lehmann.

B cells and B cell blasts withstand cryopreservation while retaining their functionality for producing antibody.

Welter A., Sundararaman S., Li R., Zhang T., Karulin A.Y., Lehmann A., Naeem V., Roen D.R., Kuerten S, and P. V. Lehmann.

High-throughput GLP-capable target cell visualization assay for measuring cell-mediated cytotoxicity.

Lehmann, A., Megyesi Z., Przybyla A., and P.V. Lehmann.

Reagent Tracker Dyes permit quality control for verifying plating accuracy in ELISPOT tests.

Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten and Paul V. Lehmann.

A positive control for detection of functional CD4 T cells in PBMC: The CPI pool.

Andrea Duechting, A., Przybyla, A., Kuerten, S. and Lehmann, P.V

Delayed activation kinetics of Th2 and Th17 vs. Th1 Cells.

Megyesi, Z., Lehmann, P.V., and Karulin A.Y.

Multi-color FLUOROSPOT counting using the ImmunoSpot® Fluoro-X Suite.

Caspell, R., and Paul V. Lehmann.

Detecting all Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assay.

Roen, D.R., Hanson J, and Lehmann, P.V.

Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.

Hanson, J. and Lehmann, P.V.

Four color ELISPOT assay for the identification of Effector T cell subpopulations.

Alexey Y. Karulin*, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann.

Multiplexing T- and B-cell FLUOROSPOT assays: Experimental validation of the multi-color ImmunoSpot® Software based on center of mass distance algorithm.

Tariq A. Bhat, Kalathil Suresh, Paul N. Bogner, Paul V Lehmann, Thomas H. Thatcher, Richard P. Phipps, Patricia J. Sime and Yasmin Thanavala.

Secondhand smoke impairs immune responses to respiratory infections.

Marie Wunsch et al, Paul V. Lehmann and Stefanie Kuerten.

The enteric nervous system is a potenital autoimmune target in multiple sclerosis.

Wunsch M, Hohmann C, Milles B, Rostermund C, Lehmann PV, Schroeter M, Bayas A, Ulzheimer J, Mäurer M, Ergün S, and Kuerten S.

The correlation between the virus- and brain antigen-specific B cell response in the blood of patients with multiple sclerosis.

Ioana Moldovan, Oleg Targoni, Wenji Zhang, and Paul V Lehmann.

How frequently are predicted peptides actually recognized by CD8 cells?

Hanson J,Sundararaman S, Caspell R, Karacsony E, Karulin AY, and Lehmann PV.

ELISPOT assays in 384-well format: Up to 30 data points with one million cells.

Karulin AY, Karacsony K, Zhang W, Targoni OS, Moldovan I, Dittrich M, Sundararaman S, and Lehmann PV.

ELISPOTs produced by CD8 and CD4 cells follow Log Normal size distribution permitting objective counting.

Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV.

Characterization of the HCMV-Specific CD4 T cell responses that are associated with protective immunity.

Karulin AY, Caspell R, Dittrich M, and Lehmann PV.

Normal distribution of CD8+ T cell-derived ELISPOT counts within replicates justifies the reliance on parametric statistics for identifying positive responses.

Wunsch M, Caspell R, Kuerten S, Lehmann PV, and Sundararaman S.

Serial measurements of apoptotic cell numbers provide better acceptance criterion for PBMC quality than a single measurement prior to the T cell assay.

Sundararaman S, Karulin AY, Ansari T, BenHamouda N, Gottwein J, Laxmanan S, Levine SM, Loffredo JT, McArdle S, Neudoerfl C, Roen D, Silina K, Welch M, and Lehmann PV.

High reproducibility of ELISPOT counts from nine different laboratories.

Kuerten S, Pommerschein G, Barth SK, Hohmann C, Miles B, Sammer FW, Duffy CE, Wunsch M, Rovituso DM, Schroeter M, Addicks K, Kaiser CC, Lehmann PV.

Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood.

Kuerten S, Wunsch M, Lehmann PV.

Longitudinal T cell-derived IFN-γ/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis.

Chenggang Jin, Roen Diana R, Lehmann PV, Kellermann Gottfried H.

An enhanced ELISPOT assay for sensitive detection of antigen-specific T cell responses to Borrelia burgdorferi.

Lehmann PV, Sundararaman S.

Chapter: When results of T cell immune monitoring match or do not match clinical outcomes of tumor vaccine trials — What more could and should we measure?

Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV.

How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?

Karulin AY, Lehmann PV.

How ELISPOT morphology reflects on the productivity and kinetics of cells’ secretory activity.

Kuerten S, Batoulis H, Recks MS, Karacsony E, Zhang W, Subbramanian R, Lehmann, PV.

Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays.

Ramachandran H, Laux J, Moldovan I, Caspell R, Lehmann PV, Subbramanian R.

Optimal thawing of cryopreserved peripheral mononuclear cells for use in high-throughput human immune monitoring studies.

Dittrich M, Lehmann PV.

Chapter: Statistical analysis of ELISPOT assays.

Lehmann PV, Zhang W.

Chapter: Unique strengths of ELISPOT for T cell diagnostics.

Zhang W, Tan J, Liu Y, Li W, Gao Q, Lehmann PV.

T and B Cell Immunity is not Essential for the Induction of Proliferative Vitreoretinopathy.

Batoulis H, Uhl M, Addicks K, Lehmann PV, Kuerten S.

The Magnitude of the Antigen-specific T Cell Response is Separated from the Severity of Spinal Cord Histopathology in Remitting-relapsing Experimental Autoimmune Encephalomyelitis.

Karulin AY, Quast S, Hesse M, Lehmann PV.

Neuroantigen-Specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages.

Nowacki T, Bettenworth D, Ross M, Heidemann J, Lehmann PV, Lügering A.

Cytomegalovirus (CMV)-specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease.

Anthony DD, Milkovich KA, Zhang W, Rodriguez B, Yonkers NL, Heeger PS, Tary-Lehmann M, Lehmann PV.

Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT.

Kuerten S, Addicks K, Lehmann PV.

Studies on the CNS histopathology of EAE and its correlation with clinical and immunological parameters.

Zhang W, Lehmann PV.

Chapter: Objective, user-independent ELISPOT data analysis based on scientifically-validated principles.

Kuerten S and Lehmann PV.

The Immune Pathogenesis of Experimental Autoimmune Encephalomyelitis – Lessons Learned for Multiple Sclerosis?

Kuerten S, Pauly R, Rottlaender A, Rodi M, Gruppe TL, Addicks K, Tary-Lehmann M, Lehmann PV.

Myelin-reactive antibodies mediate the pathology of MBP-PLP fusion protein MP4-induced EAE.

Kuerten S, Gruppe TL, Laurentius LM, Kirch C, Tary-Lehmann M, Lehmann PV, Addicks K.

Differential patterns of spinal cord pathology induced by MP4, MOG peptide 35-55, and PLP peptide 178-191 in C57BL/6 mice.

Osorio JE, Brewoo JN, Silengo SJ, Arguello J, Moldovan IR, Tary-Lehmann M, Powell TD, Livengood JA, Kinney RM, Huang CYH and Stinchcomb DT.

Efficacy of a Tetravalent Chimeric Dengue Vaccine (DENVax) in Cynomolgus Macaques.

Ramaswami B, Popescu I, Macedo C, Luo C, Shapiro R, Metes D, Chalasani G, and Randhawa PS.

The Polyomavirus BK large T-antigen-derived peptide elicits an HLA-DR promiscuous and polyfunctional CD4+ T cell response.

Butterfield LH, Palucka AK, Britten CM, Dhodapkar MV, Hakansson L, Janetzki S, Kawakami Y, Kleen TO, Lee PP, Maccalli C, Maecker HT, Maino VC, Maio M, Malyguine A, Masucci G, Pawelec G, Potter DM, Rivoltini L, Salazar LG, Schenedel DJ, Slingluff CL, Song W, Stroncek DF, Tahara H, Thurin M, Trinchieri G, van Der Burg SH, Whiteside TL, Wigginton JM, Marincola F, Khleif S, Fox BA, Disis ML.

Recommendations from the iSBTc- SITC/FDA/NCI Workshop on Immunotherapy BioMarkers.

Kuerten S, Rottlaender A, Rodi M, Velasco VB Jr, Schroeter M, Kaiser C, Addicks K, Tary-Lehmann M, Lehmann PV.

The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood.

Javeri S, Rodi M, Tary-Lehmann M, Lehmann PV, Addicks K, Kuerten S.

Involvement of brain-derived neurotrophic factor (BDNF) in MP4-induced autoimmune encephalomyelitis.

Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M.

Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer’s disease.

Kuerten S, Rodi M, Javeri S, Gruppe TL, Tary-Lehmann M, Lehmann PV, Addicks K.

Delineating the impact of neuroantigen vs genetic diversity on MP4-induced EAE of C57BL/6 and B6.129 mice.

Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S.

ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators.

Conry SJ, Milkovich KA, Yonkers NL, Rodriguez B, Bernstein HB, Asaad R, Heinzel FP, Tary-Lehmann M, Lederman MM, Anthony DD.

Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function.

Tigno-Aranjuez JT, Jaini R, Tuohy VK, Lehmann PV, Tary-Lehmann M.

Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cells.

Tigno JT, Lehmann PV, Tary-Lehmann M.

Dissociated induction of cytotoxicity and DTH by CFA and CpG (41.8)

Yonkers NL, Milkovich KA, Rodriguez B, Post AB, Asaad R, Heinzel FP, Valdez H, Tary-Lehmann M, Anthony DD.

Accessory cell dependent NK cell mediated PBMC IFN-gamma production is defective in HIV infection.

Schlingmann TR, Shive CL, Targoni OS, Tary-Lehmann M, Lehmann PV.

Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells.

Le Grice SF, Whalen CC, Hom DL, Lehmann PV, Walker CJ.

Perspective: research highlights at the Case Western Reserve University Center for AIDS Research. Learning from the past and looking toward the future.

Kuerten S, Javeri S, Tary-Lehmann M, Lehmann PV, and Angelov DN.

Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.

Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.

Kuerten S, Asaad RJ, Schoenberger SP, Lehmann PV, Tary-Lehmann M.

The TRAIL of helpless CD8+ T cells in HIV infection.

Mirano-Bascos D, Tary-Lehmann M and Landry SJ.

Antigen structure influences helper T-cell epitope dominance in the human immune re- sponse to HIV envelope glycoprotein gp120.

Kuerten S, Schlingmann T, Rajasalu T, Angelov DN, Lehmann PV, Tary-Lehmann M.

Lack of Disease Specificity Limits the Usefulness of in vitro Costimulation in HIV- and HCV-Infected Patients.

Tary-Lehmann M, Hamm CD, Lehmann PV.

Chapter: Validating reference samples for comparison in a regulated ELISPOT assay.

Hattier T and Lehmann PV.

Making the jump to high throughput microbial detection

Fanning LR, Hegerfeldt Y, Tary-Lehmann M, Lesniewski M, Maciejewski J, Weitzel RP, Kozik M, Finney M, Lazarus HM, Paul P, Ratajczak MZ, Meyerson HJ, Laughlin MJ.

Allogeneic transplantation of multiple umbilical cord blood units in adults: role of pretransplant-mixed lymphocyte reaction to predict host-vs-graft rejection.

Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.

Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice BMC.

Ott PA, Tary-Lehmann M, and Lehmann PV.

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types.

Kuerten S, Kostova-Bales DA, Frenzel LP, Tigno JT, Tary-Lehmann M, Doychin N. Angelov, Lehmann PV.

MP4- and MOG:35-55-induced EAE in C57BL/6 mice differentially targets brain, spinal cord and cerebellum.

Hofstetter HH, Toyka KV, Tary-Lehmann M, Lehmann PV.

Kinetics and Organ Distribution of IL-17-Producing CD4 Cells in Proteolipid Protein 139-151 Peptide-Induced Experimental Autoimmune Encephalomyelitis of SJL Mice.

Hofstetter HH, Kovalovski A, Shive C, Lehmann PV, Forsthuber TG.

Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood.

Nowacki TM, Kuerten S, Zhang W, Boehm BO, Lehmann PV, Tary-Lehmann M.

Granzyme-B production, but not cytokine signatures distinguish recently activated CD8 memory cells from resting memory cells.

Lichtenegger FS, Kuerten S, Faas S, Boehm BO, Tary-Lehmann M, Lehmann PV.

Dissociation of EAE protective effect and allergic side reactions in tolerization with neuroantigen.

Kuerten S, Kleen T, Assad RJ, Lehmann PV, Tary-Lehmann M.

Dissociated production of perforin, granzyme B and IFN-γ by HIV-specific CD8+ cells in HIV infection.

Yonkers NL, Rodriguez B, Post AB, Asaad R, Jones L, Lederman MM, Lehmann PV, Anthony DD.

HIV Coinfection Impairs CD28-Mediated Costimulation of Hepatitis C Virus-Specific CD8 Cells.

Kuerten S, Lichtenegger FS, Faas S, Angelov DN, Tary-Lehmann M, Lehmann PV.

MBP-PLP fusion protein-induced EAE in C57BL/6 mice.

Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.

Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors.

Hofstetter HH, Karulin AY, Forsthuber TG, Ott PA, Tary-Lehmann M, Lehmann PV.

The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice.

Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M.

Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.

Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ.

Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.

Quast S, Zhang W, Shive C, Kovalovski D, Ott PA, Herzog BA, Boehm BO, Tary-Lehmann M, Karulin AY, Lehmann PV.

IL-2 absorption affects IFN- gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.

Ott PA, Anderson MR, Tary-Lehmann M, Lehmann PV.

CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes.

Berner BR, Tary-Lehmann M, Yonkers NL, Askari AD, Lehmann PV, Anthony DD.

Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE.

Hofstetter HH, Targoni OS, Karulin AY, Forsthuber TG, Tary-Lehmann M, Lehmann PV.

Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis?

Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV.

Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls.

Lehmann PV.

Chapter: Image analysis and data management of ELISPOT assay results.

Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D’Souza MP and the ELISPOT Collaborative Study Group.

Results of an ELISPOT Proficiency Panel Conducted in 11 Laboratories Participating in International Human Immunodeficiency Virus Type 1 Vaccine trials.

Herzog BA, Ott PA, Dittrich MT, Quast S, Karulin AY, Kalbacher H, Karges W, Tary-Lehmann M, Lehmann PV, Boehm BO, Durinovic-Bello U.

Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects.

Bartholomae WC., Rininsland FH, Eisenberg JC, Boehm BO, Lehmann PV, Tary-Lehmann M.

T cell immunity induced by live, necrotic, and apoptotic tumor cells.

Darabi K, Karulin AY, Boehm BO, Hofstetter HH, Fabry Z, LaManna JC, Chavez JC, Tary-Lehmann M, Lehmann PV.

The third signal in T cell-mediated autoimmune disease?

Anthony DD, Yonkers NL, Post AB, Asaad R, Heinzel FP, Lederman MM, Lehmann PV, Valdez H.

Selective impairments in dendritic cell-associated function distinguish hepatitis C virus and HIV infection.

Kleen TO, Asaad R, Landry, Boehm BO, Tary-Lehmann M.

Tc1 effector diversity shows dissociated expression of granzyme B and interferon- gamma in HIV infection.

Ott PA, Dittrich MT, Herzog BA, Guerkov R, Gottlieb PA, Putnam AL, Durinovic-Bello I, Boehm BO, Tary-Lehmann M, Lehmann PV.

T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading.

Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, and Anthony DD.

CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.

Kadereit S, Junge GR, Kleen T, Kozik MM, Kaminski BA, um-Woods K, Fu P, Tary-Lehmann M, Laughlin MJ.

Deficient IFN-gamma expression in umbilical cord blood (UCB) T cells can be rescued by IFN-gamma-mediated increase in NFATc2 expression.

Lonsdorf AS, Kuekrek H, Stern BV, Boehm BO, Lehmann PV, Tary-Lehmann M.

Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunity.

Eisenberg JC, Czinn SJ, Garhart CA, Redline RW, Bartholomae WC, Gottwein JM, Nedrud JG, Emancipator SE, Boehm BO, Lehmann PV, Blanchard TG.

Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice.

Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK.

Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.

Hricik DE, Rodriguez V, Riley J, Bryan K, Tary-Lehmann M, Greenspan N, Dejelo C, Schulak JA, Heeger PS.

Enzyme linked immunosorbent spot (ELISPOT) assay for interferon-gamma independently predicts renal function in kidney transplant recipients.

Anthony DD, Lehmann PV.

T-cell epitope mapping using the ELISPOT approach.

Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M.

CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.

Li R, Liu T, Yoshihiro F, Tary-Lehmann M, Obrenovich M, Kuekrek H, Kang SC, Pan T, Wong BS, Medof ME, Sy MS.

On the same cell type GPI- anchored normal cellular prion and DAF protein exhibit different biological properties.

Hofstetter HH, Sewell DL, Liu F, Sandor M, Forsthuber T, Lehmann PV, Fabry Z.

Autoreactive T cells promote post-traumatic healing in the central nervous system.

Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS.

Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool.

Meierhoff G, Ott PA, Lehmann PV, Schloot NC.

Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes.

Radeke HH, Tschernig T, Karulin AY, Schumm G, Emancipator SN, Resch K, Tary-Lehmann M.

CD4+ T cells recognizing specific antigen deposited in glomeruli cause glomerulonephritis-like kidney injury.

Stern BV, Boehm BO, Tary-Lehmann M.

Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity.

Schlingmann TR, Rininsland FH, Bartholomae W, Boehm BO, Lehmann PV, Tary-Lehmann M.

Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself.

Anthony DD, Valdez H, Post AB, Carlson NL, Heeger PS, Lehmann PV.

Comprehensive determinant mapping of the hepatitis C-specific CD8 cell repertoire reveals unpredicted immune hierarchy.

Valdez, H., Carlson NL, Post AB, Asaad R, Heeger PS, Lederman MM, Lehmann PV, Anthony DD.

HIV long-term non-progressors maintain brisk CD8 T cell responses to other viral antigens.

Karulin AY, Hesse MD, Yip HC, Lehmann PV.

Indirect IL-4 pathway in type 1 immunity.

Aung H, Sherman J, Tary-Lehmann M and Toossi Z.

Production of transforming growth factor-beta 1 (TGF-β1) expression in human monocytes infected with Mycobacterium avium at a single cell level by ELISPOT assay.

Gottwein JM, Blanchard TG, Targoni OS, Eisenberg JC, Zagorski BM, Redline RW, Nedrud JG, Tary-Lehmann M, Lehmann PV, Czinn SJ.

Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund’s adjuvant by systemic immunization.

Baron ED, Heeger PS, Hricik DE, Schulak JA, Tary-Lehmann M, Stevens SR.

Immunomodulatory effect of extracorporeal photopheresis after successful treatment of resistant renal allograft rejection.

Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M.

A T cell clone's avidity is a function of its activation state.

Anderson MR, Tary-Lehmann M.

Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell-dependent, but disease susceptibility is defined by the non-T-cell compartment.

Schwander SK, Torres M, Carranza CC, Escobedo D, Tary-Lehmann M, Anderson P, Toossi Z, Ellner JJ, Rich EA, Sada E.

Pulmonary mononuclear cell responses to antigens of Mycobacterium tuberculosis in healthy household contacts of patients with active tuberculosis and healthy controls from the community.

Rininsland FH, Helms T, Asaad RJ, Boehm BO, Tary-Lehmann M.

Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity.

Heeger PS, Valujskikh A, Lehmann PV.

Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen.

Pelfrey CM, Rudick RA, Cotleur AC, Lee JC, Tary-Lehmann M, Lehmann PV.

Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production.

Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.

Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant.

Targoni OS, Baus J, Hofstetter HH, Hesse MD, Karulin AY, Boehm BO, Forsthuber TG, Lehmann PV.

Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis.

Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M.

Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients.

Lehmann PV.

Selective peptidomimetic blockers of autoantigen presentation: a novel therapeutic approach to autoimmune disease.

Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV.

Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity.

Heeger PS, Greenspan NS, Kuhlenschmidt S, Dejelo C, Hricik DE, Schulak JA, Tary-Lehmann M.

Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.

Targoni OS, Tary-Lehmann M, Lehmann PV.

Prevention of murine EAE by oral hydrolytic enzyme treatment.

Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, Radeke H, Heeger PS, Trezza RP, Heinzel FP, Forsthuber TG, Lehmann PV.

Adjuvant-guided type- 1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response.

Schwander, SK, Torres M, E. Sada, C. Carranza, E. Ramos, Tary-Lehmann M, R. S. Wallis, J. Sierra, and E. A. Rich.

Enhanced responses to Mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis.

Birnbaum G, Kotilinek L, Miller SD, Raine CS, Gao YL, Lehmann PV, Gupta RS.

Heat shock proteins and experimental autoimmune encephalomyelitis. II: environmental infection and extra-neuraxial inflammation alter the course of chronic relapsing encephalomyelitis.

Lehmann PV.

The fate of T cells in the brain: veni, vidi, vici and veni, mori.

Lehmann PV, Targoni OS, Forsthuber TG.

Shifting T-cell activation thresholds in autoimmunity and determinant spreading.

Tary-Lehmann M, Hricik DE, Justice AC, Potter NS, Heeger PS.

Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure.

Gross DM, Forsthuber T, Tary-Lehmann M, Etling C, Ito K, Nagy ZA, Field JA, Steere AC, Huber BT.

Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis.

Targoni OS Lehmann PV.

Endogenous myelin basic protein inactivates the high avidity T cell repertoire.

Matesic D, Lehmann PV, Heeger PS.

High-resolution characterization of cytokine-producing alloreactivity in naive and allograft-primed mice.

Chu RS, Targoni OS, Krieg AM, Lehmann PV, Harding CV.

CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity.

Lehmann PV, D. Matesic, G. Benichou, and Heeger PS.

Induction of T helper 2 immunity to an immunodominant allopeptide.

Tian J, Lehmann PV, and D. L. Kaufman.

Determinant spreading of T helper cell 2 (Th2) responses to pancreatic islet autoantigens.

Tian J, Clare-Salzler M, Herschenfeld A, Middleton B, Newman D, Mueller R, Arita S, Evans C, Atkinson MA, Mullen Y, Sarvetnick N, Tobin AJ, Lehmann PV, Kaufman DL.

Modulating autoimmune responses to GAD inhibits disease progression and prolongs islet graft survival in diabetes-prone mice.

Lehmann PV.

Immunomodulation by proteolytic enzymes

Tian J, Atkinson MA, Clare-Salzler M, Herschenfeld A, Forsthuber T, Lehmann PV, Kaufman DL.

Nasal administration of glutamate decarboxylase (GAD65) peptides induces Th2 responses and prevents murine insulin-dependent diabetes.

Forsthuber T, Yip HC, Lehmann PV.

Induction of TH1 and TH2 immunity in neonatal mice.

Tary-Lehmann M., Saxon A, Lehmann PV.

The human immune system in hu-PBL-SCID mice.

Tian J, Lehmann PV, Kaufman DL.

T cell cross-reactivity between coxsackievirus and glutamate decarboxylase is associated with a murine diabetes susceptibility allele.

Tary-Lehmann M, Lehmann PV, Schols D, Roncarolo MG, Saxon A.

Anti-SCID mouse reactivity shapes the human CD4+ T cell repertoire in hu-PBL-SCID chimeras.

Benichou G., Fedoseyeva E, Lehmann PV, Olson CA, Geysen HM, McMillan M, Sercarz EE.

Limited T cell response to donor MHC peptides during allograft rejection. Implications for selective immune therapy in transplantation.

Uittenbogaart CH, Anisman DJ, Tary-Lehmann M, Vollger LW, Breit TM, Van Dongen JJ, Saxon A.

The SCID mouse environment causes immunophenotypic changes in human immature T-cell lines.

Kaufman DL, Clare-Salzler M, Tian J, Forsthuber T, Ting GS, Robinson P, Atkinson MA, Sercarz EE, Tobin AJ, Lehmann PV.

Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes.

Sercarz EE, Lehmann PV, Ametani A, Benichou G, Miller A, Moudgil K.

Dominance and crypticity of T cell antigenic determinants.

Lehmann PV, Sercarz EE, Forsthuber T, Dayan CM, Gammon G.

Determinant spreading and the dynamics of the autoimmune T-cell repertoire.

Tary-Lehmann M., A. Saxon.

Human mature T cells that are anergic in vivo prevail in SCID mice reconstituted with human peripheral blood.

A. Saxon. , E Macy, K Denis, M Tary-Lehmann, O Witte, J Braun

Limited B cell repertoire in severe combined immunodeficient mice engrafted with peripheral blood mononuclear cells derived from immunodeficient or normal humans.

Lehmann PV, K. Drexler, Tary-Lehmann M, F. Falcioni, U. Hurtenbach, and Nagy ZA.

Graft-versus-host resistance induced by class II major histocompatibility complex-specific T cell clones.

Tary-Lehmann, M., A. G. Rolink, Lehmann PV, Nagy ZA, and U. Hurtenbach

Induction of graft versus host-associated immunodeficiency by CD4+ T cell clones.

Przybyła A., Zhang T., Li R., Roen D.R., Mackiewicz A., and P. V. Lehmann (2018)

Natural T cell autoreactivity to melanoma antigens: clonally expanded melanoma-antigen specific CD8 + memory T cells can be detected in healthy humans.

Lehmann PV, Forsthuber T, Miller A, Sercarz EE.

Spreading of T-cell autoimmunity to cryptic determinants of an autoantigen.

Falcioni F, Dembic Z, Mulller S, Lehmann PV, Nagy ZA.

Flexibility of the T cell repertoire. Self tolerance causes a shift of T cell receptor gene usage in response to insulin.

Lehmann PV, G. Schumm, D. Moon, U. Hurtenbach, F. Falcioni, Mulller S, and Nagy ZA.

Acute lethal graft-versus-host reaction induced by major histocompatibility complex class II-reactive T helper cell clones.

Lehmann PV, Cardinaux F, Appella E, Muller S, Falcioni F, Adorini L, Nagy ZA.

Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking.

Nagy ZA, Lehmann PV, Falcioni F, Muller S, Adorini L.

Why peptides? Their possible role in the evolution of MHC-restricted T-cell recognition.

Adorini L, Mulller S, Cardinaux F, Lehmann PV, Falcioni F, Nagy ZA.

In vivo competition between self peptides and foreign antigens in T-cell activation.

Adorini L, Sette A, Buus S, Grey HM, Darsley M, Lehmann PV, Doria G, Nagy ZA, Appella E.

Interaction of an immunodominant epitope with Ia molecules in T-cell activation.

Diego VP, Luu BW, Hofmann M, Dinh LV, Almeida M, Powell JS, Rajalingam R, Peralta JM, Kumar S, Curran JE, Sauna ZE, Kellerman R, Park Y, Key NS, Escobar MA, Huynh H, Verhagen AM, Williams-Blangero S, Lehmann PV, Maraskovsky E, Blangero J, Howard TE.

Quantitative HLA-class-II/factor VIII (FVIII) peptidomic variation in dendritic cells correlates with the immunogenic potential of therapeutic FVIII proteins in hemophilia A.