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Joseph Eiden, Gilad Gordon, Carlos Fierro, Renee Herber, Roger Aitchison, Robert Belshe, Harry Greenberg, Daniel Hoft, Yasuko Hatta, Michael J Moser, Magdalena Tary-Lehmann, Yoshihiro Kawaoka, Gabriele Neumann, Paul Radspinner, Pamuk Bilsel.

Safety and Immunogenicity of M2-Deficient, Single Replication, Live Influenza Vaccine (M2SR) in Adults.

Vaccines (Basel). 2021. 9(12): 1388.
B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants

Sharon E Frey, Jack T Stapleton, Zuhair K Ballas, Wendy L Rasmussen, Thomas M Kaufman, Tammy P Blevins, Travis L Jensen, D Huw Davies, Magdalena Tary-Lehmann, Paul Chaplin, Heather Hill, Johannes B Goll, DMID 09-0002 MVA Vaccine Study Group.

Human Antibody Responses Following Vaccinia Immunization Using Protein Microarrays and Correlation with Cell-Mediated Immunity and Antibody-Dependent Cellular Cytotoxicity Responses.

The Journal of Infectious Diseases. 2021. 224(8): 1372-82.
B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants

Carla Wolf, Sebastian Köppert, Noémi Becza, Stefanie Kuerten, Greg A. Kirchenbaum, Paul V. Lehmann.

Antibody levels poorly reflect on the frequency of memory B cells generated following SARS-CoV-2, seasonal influenza, or EBV infection.

2022 In Review.
B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants

Sebastian Köppert, Carla Wolf, Noémi Becza, Giuseppe A Sautto, Fridolin Franke, Stefanie Kuerten, Ted M Ross, Paul V Lehmann, Greg A Kirchenbaum.

Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays

Cells. 2021.10(8):1843
B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants

Alexander A. Lehmann, Greg A Kirchenbaum, Ting Zhang, Pedro A Reche, Paul V. Lehmann.

Deconvoluting the T cell response to SARS-CoV-2: specificity versus chance- and cognate cross-reactivity.

Frontiers in Immunology. 2021. 12:635942
B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants

Alexander A. Lehmann, Pedro A. Reche, Ting Zhang, Maneewan Suwansaard, and Paul. V. Lehmann.

CERI, CEF-X, and CPI: largely improved positive controls for testing antigen-specific T cell function in PBMC compared to CEF.

Cells. 2021. 10, 248

Alexander A. Lehmann, Ting Zhang, Pedro A Reche, Paul V. Lehmann.

Discordance between the predicted vs. the actually recognized CD8+ T cell epitopes of HCMV pp65 antigen and aleatory epitope dominance

Frontiers in Immunology. 2021. 11: 618428

Anna Przybyla, Alexander A. Lehmann, Ting Zhang, Jacek Mackiewicz, Łukasz Galus, Greg A. Kirchenbaum, Andrzej Mackiewicz, Paul V. Lehmann.

Functional T cell reactivity to melanocyte antigens is lost during development of malignant melanoma, but is restored with successful immunotherapy.

Cancers,13: 223 2021.

Alexander Lehmann and Paul V. Lehmann.

Aleatory epitope recognition prevails in human T cell responses?

Critical Reviews in Immunology 2020, 40: 225-235.

Fridolin Franke, Greg A. Kirchenbaum, Stefanie Kuerten and Paul V. Lehmann.

IL-21 in conjunction with anti-CD40 and IL-4 constitutes a potent polyclonal B cell stimulator for monitoring antigen-specific memory B cells.

Cells,2020, 9: 433.

Paul V. Lehmann, Maneewan Suwansaard, Ting Zhang, Diana R. Roen, Greg A. Kirchenbaum, Alexey Y. Karulin, Alexander Lehmann, and Pedro A. Reche.

Comprehensive evaluation of the expressed CD8+ T cell epitope space using high-throughput epitope mapping.

Frontiers in Immunology, 2019,10: 655.

Kirchenbaum GA, Hanson J, Roen DR, Lehmann PV.

Detection of Antigen-Specific T Cell Lineages and Effector Functions Based on Secretory Signature.

J Immunological Sci. 2019, 3, 14-20.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Cytotoxicity, Granzyme B, and Perforin ELISPOT -

Fredrik Terlutter, Tobias M. Nowacki, Richard Caspell, Ruliang Li, Krisztian Horvath, Charlotte von Gall, Stefanie Kurten, Srividya Sundararaman, and Paul V. Lehmann.

Direct detection of T and B memory lymphocytes reveals HCMV exposure that serum antibodies fail to identify.

Cells. 2018, 19, 7.

Transplantation Immunology - Infectious and Other Diseases -

Fecher P., Caspell R., Naeem, V., Karulin A.Y., Kuerten S.and P.V. Lehmann.

B cells and B cell blasts withstand cryopreservation while retaining their functionality for producing antibody.

Cells. 2018, 7, 50

Fresh versus Cryopreserved PBMC -

Welter A., Sundararaman S., Li R., Zhang T., Karulin A.Y., Lehmann A., Naeem V., Roen D.R., Kuerten S, and P. V. Lehmann.

High-throughput GLP-capable target cell visualization assay for measuring cell-mediated cytotoxicity.

Cells. 2018, 7, 35.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Tumor Immunology -

Lehmann, A., Megyesi Z., Przybyla A., and P.V. Lehmann.

Reagent Tracker Dyes permit quality control for verifying plating accuracy in ELISPOT tests.

Cells. 2018, 3, 7.

Working with Peptides and Peptide Pools -

Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten and Paul V. Lehmann.

A positive control for detection of functional CD4 T cells in PBMC: The CPI pool.

Cells. 2017, 6, 47

Working with Peptides and Peptide Pools -

Andrea Duechting, A., Przybyla, A., Kuerten, S. and Lehmann, P.V

Delayed activation kinetics of Th2 and Th17 vs. Th1 Cells.

Cells. 6, 47

Measuring Th1, Th2, Th17, and Other Effector Lineages -

Megyesi, Z., Lehmann, P.V., and Karulin A.Y.

Multi-color FLUOROSPOT counting using the ImmunoSpot® Fluoro-X Suite.

Methods in Molecular Biology. 2018, 1808, 115-132.

Caspell, R., and Paul V. Lehmann.

Detecting all Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assay.

Methods in Molecular Biology. 2018, 1808, 85-94.

Roen, D.R., Hanson J, and Lehmann, P.V.

Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.

Methods in Molecular Biology. 2018, 1808, 73-84

Hanson, J. and Lehmann, P.V.

Four color ELISPOT assay for the identification of Effector T cell subpopulations.

Methods in Molecular Biology. 2018, 1808, 51-62

Measuring Th1, Th2, Th17, and Other Effector Lineages -

Alexey Y. Karulin*, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann.

Multiplexing T- and B-cell FLUOROSPOT assays: Experimental validation of the multi-color ImmunoSpot® Software based on center of mass distance algorithm.

Methods in Molecular Biology, 3rd Edition (CH 9). 2018, 1808, 95-114.

Cognate Cytokine Production by T Cells versus Bystander Cytokine Production -

Tariq A. Bhat, Kalathil Suresh, Paul N. Bogner, Paul V Lehmann, Thomas H. Thatcher, Richard P. Phipps, Patricia J. Sime and Yasmin Thanavala.

Secondhand smoke impairs immune responses to respiratory infections.

J. Immunology. 2018, 15, 2927-2940.

Tumor Immunology -

Marie Wunsch et al, Paul V. Lehmann and Stefanie Kuerten.

The enteric nervous system is a potenital autoimmune target in multiple sclerosis.

Acta Neuropathologica. 2017, 134, 281-295.

Autoimmunity

Wunsch M, Hohmann C, Milles B, Rostermund C, Lehmann PV, Schroeter M, Bayas A, Ulzheimer J, Mäurer M, Ergün S, and Kuerten S.

The correlation between the virus- and brain antigen-specific B cell response in the blood of patients with multiple sclerosis.

Viruses. 2016, 8, 105.

AutoimmunityAutoimmunity

Ioana Moldovan, Oleg Targoni, Wenji Zhang, and Paul V Lehmann.

How frequently are predicted peptides actually recognized by CD8 cells?

Cancer Immunology, Immunotherapy. 2016, 65, 847-855.

Single-cell Resolution and Frequency - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Infectious and Other Diseases -

Hanson J,Sundararaman S, Caspell R, Karacsony E, Karulin AY, and Lehmann PV.

ELISPOT assays in 384-well format: Up to 30 data points with one million cells.

Cells. 2015, 4, 71-83.

Single-cell Resolution and Frequency -

Karulin AY, Karacsony K, Zhang W, Targoni OS, Moldovan I, Dittrich M, Sundararaman S, and Lehmann PV.

ELISPOTs produced by CD8 and CD4 cells follow Log Normal size distribution permitting objective counting.

Cells. 2015, 4, 56-70.

Spot Size Distribution -

Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV.

Characterization of the HCMV-Specific CD4 T cell responses that are associated with protective immunity.

Cells. 2015, 7, 4414-4437.

Single-cell Resolution and Frequency - Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Cytokine Coexpression/Two-color ELISPOT Assays - Spot Size Distribution - Infectious and Other Diseases -

Karulin AY, Caspell R, Dittrich M, and Lehmann PV.

Normal distribution of CD8+ T cell-derived ELISPOT counts within replicates justifies the reliance on parametric statistics for identifying positive responses.

Cells. 2015, 4, 96-111.

Single-cell Resolution and Frequency -

Wunsch M, Caspell R, Kuerten S, Lehmann PV, and Sundararaman S.

Serial measurements of apoptotic cell numbers provide better acceptance criterion for PBMC quality than a single measurement prior to the T cell assay.

Cells. 2015, 4, 40-55.

Fresh versus Cryopreserved PBMC -

Sundararaman S, Karulin AY, Ansari T, BenHamouda N, Gottwein J, Laxmanan S, Levine SM, Loffredo JT, McArdle S, Neudoerfl C, Roen D, Silina K, Welch M, and Lehmann PV.

High reproducibility of ELISPOT counts from nine different laboratories.

Cells. 2015, 4, 21-39.

Spot Size Distribution - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC -

Kuerten S, Pommerschein G, Barth SK, Hohmann C, Miles B, Sammer FW, Duffy CE, Wunsch M, Rovituso DM, Schroeter M, Addicks K, Kaiser CC, Lehmann PV.

Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood.

Clinical Immunology. 2014, 152, 20-24.

Autoimmunity

Kuerten S, Wunsch M, Lehmann PV.

Longitudinal T cell-derived IFN-γ/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis.

Journal of Immunological Methods. 2013, 398-399, 68-75.

Cytokine Coexpression/Two-color ELISPOT Assays - Autoimmunity

Chenggang Jin, Roen Diana R, Lehmann PV, Kellermann Gottfried H.

An enhanced ELISPOT assay for sensitive detection of antigen-specific T cell responses to Borrelia burgdorferi.

Cells. 2013, 2, 607-620.

Infectious and Other Diseases -

Lehmann PV, Sundararaman S.

Chapter: When results of T cell immune monitoring match or do not match clinical outcomes of tumor vaccine trials — What more could and should we measure?

The Tumor Immunoenvironment. 2013, 725-740.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Tumor Immunology -

Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV.

How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?

Viruses. 2012, 4, 2636-2649.

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Working with Peptides and Peptide Pools - Infectious and Other Diseases -

Karulin AY, Lehmann PV.

How ELISPOT morphology reflects on the productivity and kinetics of cells’ secretory activity.

Methods in Molecular Biology. 2012, 792, 125-143.

Kuerten S, Batoulis H, Recks MS, Karacsony E, Zhang W, Subbramanian R, Lehmann, PV.

Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays.

Cells. 2012, 1, 313-324.

Single-cell Resolution and Frequency - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC -

Ramachandran H, Laux J, Moldovan I, Caspell R, Lehmann PV, Subbramanian R.

Optimal thawing of cryopreserved peripheral mononuclear cells for use in high-throughput human immune monitoring studies.

Cells. 2012, 1, 313-324.

Fresh versus Cryopreserved PBMC -

Dittrich M, Lehmann PV.

Chapter: Statistical analysis of ELISPOT assays.

Methods in Molecular Biology. 2012, 792, 173-183.

Lehmann PV, Zhang W.

Chapter: Unique strengths of ELISPOT for T cell diagnostics.

Methods in Molecular Biology. 2012. 792, 3-23.

Zhang W, Tan J, Liu Y, Li W, Gao Q, Lehmann PV.

T and B Cell Immunity is not Essential for the Induction of Proliferative Vitreoretinopathy.

Eye. 2012, 26, 872-881.

Autoimmunity

Batoulis H, Uhl M, Addicks K, Lehmann PV, Kuerten S.

The Magnitude of the Antigen-specific T Cell Response is Separated from the Severity of Spinal Cord Histopathology in Remitting-relapsing Experimental Autoimmune Encephalomyelitis.

Glia. 2012, 60, 794-805.

Autoimmunity

Karulin AY, Quast S, Hesse M, Lehmann PV.

Neuroantigen-Specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages.

Cells. 2012, 1, 576-596.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Autoimmunity

Nowacki T, Bettenworth D, Ross M, Heidemann J, Lehmann PV, Lügering A.

Cytomegalovirus (CMV)-specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease.

Cells. 2012, 1, 35-50.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

Anthony DD, Milkovich KA, Zhang W, Rodriguez B, Yonkers NL, Heeger PS, Tary-Lehmann M, Lehmann PV.

Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT.

Cells. 2012, 1, 127-140.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production -

Kuerten S, Addicks K, Lehmann PV.

Studies on the CNS histopathology of EAE and its correlation with clinical and immunological parameters.

Cells. 2012, 1, 576-596.

Autoimmunity

Zhang W, Lehmann PV.

Chapter: Objective, user-independent ELISPOT data analysis based on scientifically-validated principles.

Methods and Protocols, Handbook of ELISPOT. 2011, 792, 155-171.

Kuerten S and Lehmann PV.

The Immune Pathogenesis of Experimental Autoimmune Encephalomyelitis – Lessons Learned for Multiple Sclerosis?

Journal of Interferon & Cytokine Research. 2011. 31:907-16.

Autoimmunity

Kuerten S, Pauly R, Rottlaender A, Rodi M, Gruppe TL, Addicks K, Tary-Lehmann M, Lehmann PV.

Myelin-reactive antibodies mediate the pathology of MBP-PLP fusion protein MP4-induced EAE.

Clinical Immunology. 2011, 140, 54-62.

Working with Peptides and Peptide Pools - Autoimmunity

Kuerten S, Gruppe TL, Laurentius LM, Kirch C, Tary-Lehmann M, Lehmann PV, Addicks K.

Differential patterns of spinal cord pathology induced by MP4, MOG peptide 35-55, and PLP peptide 178-191 in C57BL/6 mice.

APMIS. 2011, 119, 336-346.

Autoimmunity

Osorio JE, Brewoo JN, Silengo SJ, Arguello J, Moldovan IR, Tary-Lehmann M, Powell TD, Livengood JA, Kinney RM, Huang CYH and Stinchcomb DT.

Efficacy of a Tetravalent Chimeric Dengue Vaccine (DENVax) in Cynomolgus Macaques.

American Society of Tropical Medicine and Hygiene. 84(6), 2011. 978–987.

Infectious and Other Diseases -

Ramaswami B, Popescu I, Macedo C, Luo C, Shapiro R, Metes D, Chalasani G, and Randhawa PS.

The Polyomavirus BK large T-antigen-derived peptide elicits an HLA-DR promiscuous and polyfunctional CD4+ T cell response.

Clinical Vaccine Immunology. 2011, 18, 815-824.

Infectious and Other Diseases -

Butterfield LH, Palucka AK, Britten CM, Dhodapkar MV, Hakansson L, Janetzki S, Kawakami Y, Kleen TO, Lee PP, Maccalli C, Maecker HT, Maino VC, Maio M, Malyguine A, Masucci G, Pawelec G, Potter DM, Rivoltini L, Salazar LG, Schenedel DJ, Slingluff CL, Song W, Stroncek DF, Tahara H, Thurin M, Trinchieri G, van Der Burg SH, Whiteside TL, Wigginton JM, Marincola F, Khleif S, Fox BA, Disis ML.

Recommendations from the iSBTc- SITC/FDA/NCI Workshop on Immunotherapy BioMarkers.

Clinical Cancer Research. 2011, 17, 3064-3076.

Tumor Immunology -

Kuerten S, Rottlaender A, Rodi M, Velasco VB Jr, Schroeter M, Kaiser C, Addicks K, Tary-Lehmann M, Lehmann PV.

The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood.

Clinical Immunology. 2010, 137, 422-432.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Autoimmunity

Javeri S, Rodi M, Tary-Lehmann M, Lehmann PV, Addicks K, Kuerten S.

Involvement of brain-derived neurotrophic factor (BDNF) in MP4-induced autoimmune encephalomyelitis.

Clinical Immunology. 2010, 137, 181-189.

Autoimmunity

Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M.

Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer’s disease.

Neurobiology of Aging. 2010, 31, 1732-42.

Infectious and Other Diseases -

Kuerten S, Rodi M, Javeri S, Gruppe TL, Tary-Lehmann M, Lehmann PV, Addicks K.

Delineating the impact of neuroantigen vs genetic diversity on MP4-induced EAE of C57BL/6 and B6.129 mice.

APMIS. 2009, 117, 923-35.

Autoimmunity

Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S.

ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators.

Journal of Immunotoxicology. 2009, 6, 227-34.

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC -

Conry SJ, Milkovich KA, Yonkers NL, Rodriguez B, Bernstein HB, Asaad R, Heinzel FP, Tary-Lehmann M, Lederman MM, Anthony DD.

Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function.

Journal of Virology. 2009, 83, 11175-11187.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Tigno-Aranjuez JT, Jaini R, Tuohy VK, Lehmann PV, Tary-Lehmann M.

Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cells.

Journal of Immunology. 2009, 183, 5654-5661.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Vaccine and Adjuvants - Autoimmunity

Tigno JT, Lehmann PV, Tary-Lehmann M.

Dissociated induction of cytotoxicity and DTH by CFA and CpG (41.8)

Journal of Immunotherapy. 2009, 32, 389.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytotoxicity, Granzyme B, and Perforin ELISPOT - Vaccine and Adjuvants -

Yonkers NL, Milkovich KA, Rodriguez B, Post AB, Asaad R, Heinzel FP, Valdez H, Tary-Lehmann M, Anthony DD.

Accessory cell dependent NK cell mediated PBMC IFN-gamma production is defective in HIV infection.

Clinical Immunology. 2009, 131, 288-297.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Schlingmann TR, Shive CL, Targoni OS, Tary-Lehmann M, Lehmann PV.

Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells.

Cellular Immunology. 2009, 258, 131-137.

Spot Size Distribution -

Le Grice SF, Whalen CC, Hom DL, Lehmann PV, Walker CJ.

Perspective: research highlights at the Case Western Reserve University Center for AIDS Research. Learning from the past and looking toward the future.

AIDS Research and Human Retroviruses. 1996, 12, 1659.

Kuerten S, Javeri S, Tary-Lehmann M, Lehmann PV, and Angelov DN.

Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.

Infectious and Other Diseases -

Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.

Clinical Immunology. 2008, 129, 256-267.

Autoimmunity

Kuerten S, Asaad RJ, Schoenberger SP, Lehmann PV, Tary-Lehmann M.

The TRAIL of helpless CD8+ T cells in HIV infection.

AIDS Research and Human Retroviruses. 2008, 24, 1-9.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Infectious and Other Diseases -

Mirano-Bascos D, Tary-Lehmann M and Landry SJ.

Antigen structure influences helper T-cell epitope dominance in the human immune re- sponse to HIV envelope glycoprotein gp120.

European Journal of Immunology. 2008, 38, 1231-1237.

Infectious and Other Diseases -

Kuerten S, Schlingmann T, Rajasalu T, Angelov DN, Lehmann PV, Tary-Lehmann M.

Lack of Disease Specificity Limits the Usefulness of in vitro Costimulation in HIV- and HCV-Infected Patients.

Clinical and Developmental Immunology. 2008, 38, 1231-1237.

Infectious and Other Diseases -

Tary-Lehmann M, Hamm CD, Lehmann PV.

Chapter: Validating reference samples for comparison in a regulated ELISPOT assay.

Validation of Cell-based Assays in the GLP Setting. (CH 9) 2008.

Hattier T and Lehmann PV.

Making the jump to high throughput microbial detection

Medlab. 2008, 3, 30-32.

Autoimmunity

Fanning LR, Hegerfeldt Y, Tary-Lehmann M, Lesniewski M, Maciejewski J, Weitzel RP, Kozik M, Finney M, Lazarus HM, Paul P, Ratajczak MZ, Meyerson HJ, Laughlin MJ.

Allogeneic transplantation of multiple umbilical cord blood units in adults: role of pretransplant-mixed lymphocyte reaction to predict host-vs-graft rejection.

Leukemia. 2008, 22, 1786-90.

Transplantation Immunology -

Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.

Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice BMC.

Musculoskeletal Disorders. 2006. 7:32.

Transplantation Immunology -

Ott PA, Tary-Lehmann M, and Lehmann PV.

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types.

Clinical Immunology. 2007, 124, 267-276.

Single-cell Resolution and Frequency -

Kuerten S, Kostova-Bales DA, Frenzel LP, Tigno JT, Tary-Lehmann M, Doychin N. Angelov, Lehmann PV.

MP4- and MOG:35-55-induced EAE in C57BL/6 mice differentially targets brain, spinal cord and cerebellum.

Journal of Neuroimmunology. 2007, 189, 31-40.

Autoimmunity

Hofstetter HH, Toyka KV, Tary-Lehmann M, Lehmann PV.

Kinetics and Organ Distribution of IL-17-Producing CD4 Cells in Proteolipid Protein 139-151 Peptide-Induced Experimental Autoimmune Encephalomyelitis of SJL Mice.

Journal of Immunology. 2007, 178, 1372-1378.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Hofstetter HH, Kovalovski A, Shive C, Lehmann PV, Forsthuber TG.

Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood.

Journal of Neuroimmunology. 2007, 187, 20-30.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Nowacki TM, Kuerten S, Zhang W, Boehm BO, Lehmann PV, Tary-Lehmann M.

Granzyme-B production, but not cytokine signatures distinguish recently activated CD8 memory cells from resting memory cells.

Cell Immunology. 2007, 247, 36-48.

Cytotoxicity, Granzyme B, and Perforin ELISPOT -

Lichtenegger FS, Kuerten S, Faas S, Boehm BO, Tary-Lehmann M, Lehmann PV.

Dissociation of EAE protective effect and allergic side reactions in tolerization with neuroantigen.

Journal of Immunology. 2006, 178, 4749-4756.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Kuerten S, Kleen T, Assad RJ, Lehmann PV, Tary-Lehmann M.

Dissociated production of perforin, granzyme B and IFN-γ by HIV-specific CD8+ cells in HIV infection.

AIDS Research and Human Retroviruses. 2007, 24, 62-71.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

Yonkers NL, Rodriguez B, Post AB, Asaad R, Jones L, Lederman MM, Lehmann PV, Anthony DD.

HIV Coinfection Impairs CD28-Mediated Costimulation of Hepatitis C Virus-Specific CD8 Cells.

Journal of Infectious Diseases. 2006, 194, 391-400.

Infectious and Other Diseases -

Kuerten S, Lichtenegger FS, Faas S, Angelov DN, Tary-Lehmann M, Lehmann PV.

MBP-PLP fusion protein-induced EAE in C57BL/6 mice.

Journal of Neuroimmunology. 2006, 177, 99-111.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.

Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors.

BMC Musculoskeletal Disorders. 2005, 36.

Transplantation Immunology -

Hofstetter HH, Karulin AY, Forsthuber TG, Ott PA, Tary-Lehmann M, Lehmann PV.

The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice.

Journal of Neuroimmunology. 2005, 170, 105-114.

Single-cell Resolution and Frequency - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Autoimmunity

Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M.

Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.

AIDS. 2005, 19, 1967-1974.

Vaccine and Adjuvants - Infectious and Other Diseases -

Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ.

Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.

Transplantation. 2005, 80, 1316-1322.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -

Quast S, Zhang W, Shive C, Kovalovski D, Ott PA, Herzog BA, Boehm BO, Tary-Lehmann M, Karulin AY, Lehmann PV.

IL-2 absorption affects IFN- gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.

Cell Immunology. 2005, 237, 28-36.

Cytokine Coexpression/Two-color ELISPOT Assays -

Ott PA, Anderson MR, Tary-Lehmann M, Lehmann PV.

CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes.

Cell Immunology. 2005, 235, 1-11.

Autoimmunity

Berner BR, Tary-Lehmann M, Yonkers NL, Askari AD, Lehmann PV, Anthony DD.

Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE.

Cell Immunology. 2005, 235, 29-38.

Infectious and Other Diseases -

Hofstetter HH, Targoni OS, Karulin AY, Forsthuber TG, Tary-Lehmann M, Lehmann PV.

Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis?

Journal of Immunology. 2005, 174, 4598-4605.

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Autoimmunity

Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV.

Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls.

Clinical Immunology. 2005, 115, 102-114.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Working with Peptides and Peptide Pools - Autoimmunity

Lehmann PV.

Chapter: Image analysis and data management of ELISPOT assay results.

2005, 302, 117-131.

Spot Size Distribution -

Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D’Souza MP and the ELISPOT Collaborative Study Group.

Results of an ELISPOT Proficiency Panel Conducted in 11 Laboratories Participating in International Human Immunodeficiency Virus Type 1 Vaccine trials.

AIDS Research & Human Retroviruses. 2005, 21, 68-81.

Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -

Herzog BA, Ott PA, Dittrich MT, Quast S, Karulin AY, Kalbacher H, Karges W, Tary-Lehmann M, Lehmann PV, Boehm BO, Durinovic-Bello U.

Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects.

Journal of Autoimmunity. 2004, 23, 45-54.

Working with Peptides and Peptide Pools - Autoimmunity

Bartholomae WC., Rininsland FH, Eisenberg JC, Boehm BO, Lehmann PV, Tary-Lehmann M.

T cell immunity induced by live, necrotic, and apoptotic tumor cells.

Journal of Immunology. 2004, 173, 1012-1022.

Tumor Immunology -

Darabi K, Karulin AY, Boehm BO, Hofstetter HH, Fabry Z, LaManna JC, Chavez JC, Tary-Lehmann M, Lehmann PV.

The third signal in T cell-mediated autoimmune disease?

Journal of Immunology. 2004, 173, 92-99.

Autoimmunity

Anthony DD, Yonkers NL, Post AB, Asaad R, Heinzel FP, Lederman MM, Lehmann PV, Valdez H.

Selective impairments in dendritic cell-associated function distinguish hepatitis C virus and HIV infection.

Journal of Immunology. 2004, 172, 4907-4916.

Infectious and Other Diseases -

Kleen TO, Asaad R, Landry, Boehm BO, Tary-Lehmann M.

Tc1 effector diversity shows dissociated expression of granzyme B and interferon- gamma in HIV infection.

AIDS. 2004, 18, 383-392.

Working with Peptides and Peptide Pools - Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

Ott PA, Dittrich MT, Herzog BA, Guerkov R, Gottlieb PA, Putnam AL, Durinovic-Bello I, Boehm BO, Tary-Lehmann M, Lehmann PV.

T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading.

J Clin Immunol. 2004, 24, 327-339.

Working with Peptides and Peptide Pools - Autoimmunity

Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, and Anthony DD.

CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.

Journal of Immunological Methods. 2004, 285, 223-235.

Working with Peptides and Peptide Pools - Vaccine and Adjuvants -

Kadereit S, Junge GR, Kleen T, Kozik MM, Kaminski BA, um-Woods K, Fu P, Tary-Lehmann M, Laughlin MJ.

Deficient IFN-gamma expression in umbilical cord blood (UCB) T cells can be rescued by IFN-gamma-mediated increase in NFATc2 expression.

Journal of Clinical Immunology. 2003, 23, 485-497.

Transplantation Immunology -

Lonsdorf AS, Kuekrek H, Stern BV, Boehm BO, Lehmann PV, Tary-Lehmann M.

Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunity.

Journal of Immunology. 2003, 171, 3941-3946.

Vaccine and Adjuvants - Tumor Immunology -

Eisenberg JC, Czinn SJ, Garhart CA, Redline RW, Bartholomae WC, Gottwein JM, Nedrud JG, Emancipator SE, Boehm BO, Lehmann PV, Blanchard TG.

Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice.

Infection and Immununity. 2003, 71, 1820-1827

Measuring Th1, Th2, Th17, and Other Effector Lineages - Vaccine and Adjuvants - Infectious and Other Diseases -

Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK.

Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.

Journal of Immunological Methods. 2003, 279, 111-121.

Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Measuring Th1, Th2, Th17, and Other Effector Lineages - Infectious and Other Diseases - Spot Size Distribution -

Hricik DE, Rodriguez V, Riley J, Bryan K, Tary-Lehmann M, Greenspan N, Dejelo C, Schulak JA, Heeger PS.

Enzyme linked immunosorbent spot (ELISPOT) assay for interferon-gamma independently predicts renal function in kidney transplant recipients.

American Journal of Transplantation. 2003, 3, 878-884.

Transplantation Immunology -

Anthony DD, Lehmann PV.

T-cell epitope mapping using the ELISPOT approach.

Methods. 2003, 29, 260-269.

Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -

Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M.

CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.

Journal of Immunological Methods. 2003, 278, 79-93.

Working with Peptides and Peptide Pools - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC -

Li R, Liu T, Yoshihiro F, Tary-Lehmann M, Obrenovich M, Kuekrek H, Kang SC, Pan T, Wong BS, Medof ME, Sy MS.

On the same cell type GPI- anchored normal cellular prion and DAF protein exhibit different biological properties.

Biochemical and Biophysical Research Communications. 2003, 303, 446-451

Infectious and Other Diseases -

Hofstetter HH, Sewell DL, Liu F, Sandor M, Forsthuber T, Lehmann PV, Fabry Z.

Autoreactive T cells promote post-traumatic healing in the central nervous system.

Journal of Neuroimmunology. 2003, 134, 25-34.

Autoimmunity

Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS.

Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool.

American Journal of Transplantation. 2002, 2, 857.

Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC - ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Transplantation Immunology -

Meierhoff G, Ott PA, Lehmann PV, Schloot NC.

Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes.

Diabetes/Metabolism Research and Reviews. 2002, 18, 367-380.

Autoimmunity

Radeke HH, Tschernig T, Karulin AY, Schumm G, Emancipator SN, Resch K, Tary-Lehmann M.

CD4+ T cells recognizing specific antigen deposited in glomeruli cause glomerulonephritis-like kidney injury.

Clinical Immunology. 2002, 104, 161-173.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Stern BV, Boehm BO, Tary-Lehmann M.

Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity.

Journal of Immunology. 2002, 168, 6099-6105.

Working with Peptides and Peptide Pools - Vaccine and Adjuvants - Tumor Immunology -

Schlingmann TR, Rininsland FH, Bartholomae W, Boehm BO, Lehmann PV, Tary-Lehmann M.

Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself.

Clinical Immunology. 2009, 133, 45-51.

Tumor Immunology -

Anthony DD, Valdez H, Post AB, Carlson NL, Heeger PS, Lehmann PV.

Comprehensive determinant mapping of the hepatitis C-specific CD8 cell repertoire reveals unpredicted immune hierarchy.

Clinical Immunology. 2002, 103, 264-276.

Determinant Mapping/High-throughput Screening - Infectious and Other Diseases -

Valdez, H., Carlson NL, Post AB, Asaad R, Heeger PS, Lederman MM, Lehmann PV, Anthony DD.

HIV long-term non-progressors maintain brisk CD8 T cell responses to other viral antigens.

AIDS. 2002, 16, 1113-1118.

Infectious and Other Diseases -

Karulin AY, Hesse MD, Yip HC, Lehmann PV.

Indirect IL-4 pathway in type 1 immunity.

Journal of Immunology. 2002, 168, 545-553.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Cytokine Coexpression/Two-color ELISPOT Assays -

Aung H, Sherman J, Tary-Lehmann M and Toossi Z.

Production of transforming growth factor-beta 1 (TGF-β1) expression in human monocytes infected with Mycobacterium avium at a single cell level by ELISPOT assay.

Journal of Immunological Methods. 2002, 259, 25-32.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Gottwein JM, Blanchard TG, Targoni OS, Eisenberg JC, Zagorski BM, Redline RW, Nedrud JG, Tary-Lehmann M, Lehmann PV, Czinn SJ.

Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund’s adjuvant by systemic immunization.

Journal of Infectious Diseases. 2001, 184, 308-314.

Vaccine and Adjuvants - Infectious and Other Diseases -

Baron ED, Heeger PS, Hricik DE, Schulak JA, Tary-Lehmann M, Stevens SR.

Immunomodulatory effect of extracorporeal photopheresis after successful treatment of resistant renal allograft rejection.

Photodermatology, Photoimmunology & Photomedicine. 2001, 17, 79-82.

Transplantation Immunology -

Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M.

A T cell clone's avidity is a function of its activation state.

Journal of Immunology. 2001, 167, 1353-1361

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Spot Size Distribution - Working with Peptides and Peptide Pools -

Anderson MR, Tary-Lehmann M.

Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell-dependent, but disease susceptibility is defined by the non-T-cell compartment.

Clinical Immunology. 2001, 98, 85-94.

Infectious and Other Diseases -

Schwander SK, Torres M, Carranza CC, Escobedo D, Tary-Lehmann M, Anderson P, Toossi Z, Ellner JJ, Rich EA, Sada E.

Pulmonary mononuclear cell responses to antigens of Mycobacterium tuberculosis in healthy household contacts of patients with active tuberculosis and healthy controls from the community.

Journal of Immunology. 2000, 165, 1479-1485.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Rininsland FH, Helms T, Asaad RJ, Boehm BO, Tary-Lehmann M.

Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity.

Journal of Immunological Methods. 2000, 240, 143-155.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

Heeger PS, Valujskikh A, Lehmann PV.

Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen.

Journal of Immunology. 2000, 165, 12781284.

Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Transplantation Immunology -

Pelfrey CM, Rudick RA, Cotleur AC, Lee JC, Tary-Lehmann M, Lehmann PV.

Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production.

Journal of Immunology. 2000, 165, 1641-1651.

Working with Peptides and Peptide Pools - Autoimmunity

Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.

Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant.

Journal of Immunology. 2000, 164, 5771-5781.

Cytokine Coexpression/Two-color ELISPOT Assays - Vaccine and Adjuvants - AutoimmunityTransplantation Immunology -

Targoni OS, Baus J, Hofstetter HH, Hesse MD, Karulin AY, Boehm BO, Forsthuber TG, Lehmann PV.

Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis.

Journal of Immunology. 2001, 166, 4757-4764.

Autoimmunity Single-cell Resolution and Frequency -

Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M.

Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients.

Journal of Immunology. 2000, 164, 3723-3732

Single-cell Resolution and Frequency - Measuring Th1, Th2, Th17, and Other Effector Lineages - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -

Lehmann PV.

Selective peptidomimetic blockers of autoantigen presentation: a novel therapeutic approach to autoimmune disease.

Trends in Pharmacological Sciences. 2000, 21, 79-80.

Autoimmunity

Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV.

Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity.

Journal of Immunology. 2000, 164, 1862-1872.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Spot Size Distribution -

Heeger PS, Greenspan NS, Kuhlenschmidt S, Dejelo C, Hricik DE, Schulak JA, Tary-Lehmann M.

Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.

Journal of Immunology. 1999, 163, 2267-2285.

Transplantation Immunology -

Targoni OS, Tary-Lehmann M, Lehmann PV.

Prevention of murine EAE by oral hydrolytic enzyme treatment.

Journal of Autoimmunity. 1999, 12, 191-198.

Autoimmunity

Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, Radeke H, Heeger PS, Trezza RP, Heinzel FP, Forsthuber TG, Lehmann PV.

Adjuvant-guided type- 1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response.

Journal of Immunology. 1999, 162, 3942-3949.

Determinant Mapping/High-throughput Screening - Vaccine and Adjuvants - Measuring Th1, Th2, Th17, and Other Effector Lineages - Measurements of Functional T Cell Avidity -

Schwander, SK, Torres M, E. Sada, C. Carranza, E. Ramos, Tary-Lehmann M, R. S. Wallis, J. Sierra, and E. A. Rich.

Enhanced responses to Mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis.

Journal of Infectious Diseases. 1998, 178, 1434-1445.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Birnbaum G, Kotilinek L, Miller SD, Raine CS, Gao YL, Lehmann PV, Gupta RS.

Heat shock proteins and experimental autoimmune encephalomyelitis. II: environmental infection and extra-neuraxial inflammation alter the course of chronic relapsing encephalomyelitis.

Journal of Neuroimmunology. 1998, 90, 149-161.

Autoimmunity

Lehmann PV.

The fate of T cells in the brain: veni, vidi, vici and veni, mori.

American Journal of Pathology. 1998, 153, 677-680.

Autoimmunity

Lehmann PV, Targoni OS, Forsthuber TG.

Shifting T-cell activation thresholds in autoimmunity and determinant spreading.

Immunological Review. 1998, 164, 53-61.

Autoimmunity

Tary-Lehmann M, Hricik DE, Justice AC, Potter NS, Heeger PS.

Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure.

Transplantation. 1998, 66, 219.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -

Gross DM, Forsthuber T, Tary-Lehmann M, Etling C, Ito K, Nagy ZA, Field JA, Steere AC, Huber BT.

Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis.

Science. 1998, 281, 703-706.

AutoimmunityInfectious and Other Diseases -

Targoni OS Lehmann PV.

Endogenous myelin basic protein inactivates the high avidity T cell repertoire.

Journal of Experimental Medicine. 1998, 187, 2055-2063.

Measurements of Functional T Cell Avidity - Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Autoimmunity

Matesic D, Lehmann PV, Heeger PS.

High-resolution characterization of cytokine-producing alloreactivity in naive and allograft-primed mice.

Transplantation. 1998, 65, 906-914.

Transplantation Immunology -

Chu RS, Targoni OS, Krieg AM, Lehmann PV, Harding CV.

CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity.

Journal of Experimental Medicine. 1997, 186, 1623-1631.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Working with Peptides and Peptide Pools - Vaccine and Adjuvants -

Lehmann PV, D. Matesic, G. Benichou, and Heeger PS.

Induction of T helper 2 immunity to an immunodominant allopeptide.

Transplantation. 1997, 64, 292-296.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -

Tian J, Lehmann PV, and D. L. Kaufman.

Determinant spreading of T helper cell 2 (Th2) responses to pancreatic islet autoantigens.

Journal of Experimental Medicine. 1997, 186, 2039-2043.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Tian J, Clare-Salzler M, Herschenfeld A, Middleton B, Newman D, Mueller R, Arita S, Evans C, Atkinson MA, Mullen Y, Sarvetnick N, Tobin AJ, Lehmann PV, Kaufman DL.

Modulating autoimmune responses to GAD inhibits disease progression and prolongs islet graft survival in diabetes-prone mice.

Nature Medicine. 1996, 2, 1348-1353.

Autoimmunity

Lehmann PV.

Immunomodulation by proteolytic enzymes

Nephrology Dialysis Transplantation. 1996, 11, 952-955.

Transplantation Immunology -

Tian J, Atkinson MA, Clare-Salzler M, Herschenfeld A, Forsthuber T, Lehmann PV, Kaufman DL.

Nasal administration of glutamate decarboxylase (GAD65) peptides induces Th2 responses and prevents murine insulin-dependent diabetes.

Journal of Experimental Medicine. 1996, 183, 1561-1567

Autoimmunity

Forsthuber T, Yip HC, Lehmann PV.

Induction of TH1 and TH2 immunity in neonatal mice.

Science. 1996, 271, 1728-1730.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Working with Peptides and Peptide Pools - Vaccine and Adjuvants -

Tary-Lehmann M., Saxon A, Lehmann PV.

The human immune system in hu-PBL-SCID mice.

Immunology Today. 1995, 16, 529-533.

Transplantation Immunology -

Tian J, Lehmann PV, Kaufman DL.

T cell cross-reactivity between coxsackievirus and glutamate decarboxylase is associated with a murine diabetes susceptibility allele.

Journal of Experimental Medicine. 1994, 180, 1979-1984.

Autoimmunity

Tary-Lehmann M, Lehmann PV, Schols D, Roncarolo MG, Saxon A.

Anti-SCID mouse reactivity shapes the human CD4+ T cell repertoire in hu-PBL-SCID chimeras.

Journal of Experimental Medicine. 1994, 180,1817-1827.

Transplantation Immunology -

Benichou G., Fedoseyeva E, Lehmann PV, Olson CA, Geysen HM, McMillan M, Sercarz EE.

Limited T cell response to donor MHC peptides during allograft rejection. Implications for selective immune therapy in transplantation.

Journal of Immunology. 1994, 153, 938-945.

Transplantation Immunology -

Uittenbogaart CH, Anisman DJ, Tary-Lehmann M, Vollger LW, Breit TM, Van Dongen JJ, Saxon A.

The SCID mouse environment causes immunophenotypic changes in human immature T-cell lines.

International Journal of Cancer. 1994, 56, 546-551.

Tumor Immunology -

Kaufman DL, Clare-Salzler M, Tian J, Forsthuber T, Ting GS, Robinson P, Atkinson MA, Sercarz EE, Tobin AJ, Lehmann PV.

Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes.

Nature. 1993, 366, 69-72.

Autoimmunity

Sercarz EE, Lehmann PV, Ametani A, Benichou G, Miller A, Moudgil K.

Dominance and crypticity of T cell antigenic determinants.

Annual Review of Immunology. 1993, 11, 729-766.

Autoimmunity

Lehmann PV, Sercarz EE, Forsthuber T, Dayan CM, Gammon G.

Determinant spreading and the dynamics of the autoimmune T-cell repertoire.

Immunology Today. 1993, 14, 203-208.

Autoimmunity

Tary-Lehmann M., A. Saxon.

Human mature T cells that are anergic in vivo prevail in SCID mice reconstituted with human peripheral blood.

Journal of Experimental Medicine. 1992, 175, 503-516.

Transplantation Immunology -

A. Saxon. , E Macy, K Denis, M Tary-Lehmann, O Witte, J Braun

Limited B cell repertoire in severe combined immunodeficient mice engrafted with peripheral blood mononuclear cells derived from immunodeficient or normal humans.

The Journal of Clinical Investigation. 1991, 87, 658-665.

Transplantation Immunology -

Lehmann PV, K. Drexler, Tary-Lehmann M, F. Falcioni, U. Hurtenbach, and Nagy ZA.

Graft-versus-host resistance induced by class II major histocompatibility complex-specific T cell clones.

Journal of Experimental Medicine. 1991, 173, 333-341.

Transplantation Immunology -

Tary-Lehmann, M., A. G. Rolink, Lehmann PV, Nagy ZA, and U. Hurtenbach

Induction of graft versus host-associated immunodeficiency by CD4+ T cell clones.

Journal of Immunology. 1990, 145, 2092-2098.

Transplantation Immunology

Przybyła A., Zhang T., Li R., Roen D.R., Mackiewicz A., and P. V. Lehmann (2018)

Natural T cell autoreactivity to melanoma antigens: clonally expanded melanoma-antigen specific CD8 + memory T cells can be detected in healthy humans.

Cancer Immunol. Immunotherapy 2019

Lehmann PV, Forsthuber T, Miller A, Sercarz EE.

Spreading of T-cell autoimmunity to cryptic determinants of an autoantigen.

Nature. 1992, 358, 155.

Autoimmunity

Falcioni F, Dembic Z, Mulller S, Lehmann PV, Nagy ZA.

Flexibility of the T cell repertoire. Self tolerance causes a shift of T cell receptor gene usage in response to insulin.

Journal of Experimental Medicine. 1990, 171, 1665-1681.

Autoimmunity

Lehmann PV, G. Schumm, D. Moon, U. Hurtenbach, F. Falcioni, Mulller S, and Nagy ZA.

Acute lethal graft-versus-host reaction induced by major histocompatibility complex class II-reactive T helper cell clones.

Journal of Experimental Medicine. 1990, 171, 1485-1496.

Transplantation Immunology -

Lehmann PV, Cardinaux F, Appella E, Muller S, Falcioni F, Adorini L, Nagy ZA.

Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking.

European Journal of Immunology. 1989, 19, 1071-1077.

Working with Peptides and Peptide Pools -

Nagy ZA, Lehmann PV, Falcioni F, Muller S, Adorini L.

Why peptides? Their possible role in the evolution of MHC-restricted T-cell recognition.

Immunology Today. 1989, 10, 132-138

Working with Peptides and Peptide Pools -

Adorini L, Mulller S, Cardinaux F, Lehmann PV, Falcioni F, Nagy ZA.

In vivo competition between self peptides and foreign antigens in T-cell activation.

Nature. 1988, 334, 623-625.

Working with Peptides and Peptide Pools -

Adorini L, Sette A, Buus S, Grey HM, Darsley M, Lehmann PV, Doria G, Nagy ZA, Appella E.

Interaction of an immunodominant epitope with Ia molecules in T-cell activation.

Proceedings of the National Academy of Sciences. 1988, 85, 5181-5185.

Working with Peptides and Peptide Pools

Diego VP, Luu BW, Hofmann M, Dinh LV, Almeida M, Powell JS, Rajalingam R, Peralta JM, Kumar S, Curran JE, Sauna ZE, Kellerman R, Park Y, Key NS, Escobar MA, Huynh H, Verhagen AM, Williams-Blangero S, Lehmann PV, Maraskovsky E, Blangero J, Howard TE.

Quantitative HLA-class-II/factor VIII (FVIII) peptidomic variation in dendritic cells correlates with the immunogenic potential of therapeutic FVIII proteins in hemophilia A.

J Thromb Haemost 2020,18, 201-216.

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Single-cell Resolution and Frequency
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Measurements of Functional T Cell Avidity
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Spot Size Distribution
Determinant Mapping/High-throughput Screening
Working with Peptides and Peptide Pools
Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC
Fresh versus Cryopreserved PBMC
Cytotoxicity, Granzyme B, and Perforin ELISPOT
ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells
Vaccine and Adjuvants
Autoimmunity
Tumor Immunology
Transplantation Immunology
Infectious and Other Diseases
B-cell ELISPOT
ELISPOT-related Reviews/Book Chapters