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Safety and Immunogenicity of M2-Deficient, Single Replication, Live Influenza Vaccine (M2SR) in Adults.
Vaccines (Basel). 2021. 9(12): 1388.B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants
Human Antibody Responses Following Vaccinia Immunization Using Protein Microarrays and Correlation with Cell-Mediated Immunity and Antibody-Dependent Cellular Cytotoxicity Responses.
The Journal of Infectious Diseases. 2021. 224(8): 1372-82.B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants
Antibody levels poorly reflect on the frequency of memory B cells generated following SARS-CoV-2, seasonal influenza, or EBV infection.
2022 In Review.B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants
Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays
Cells. 2021.10(8):1843B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants
Deconvoluting the T cell response to SARS-CoV-2: specificity versus chance- and cognate cross-reactivity.
Frontiers in Immunology. 2021. 12:635942B Cell ELISPOT - Infectious and Other Diseases - Vaccine and Adjuvants
CERI, CEF-X, and CPI: largely improved positive controls for testing antigen-specific T cell function in PBMC compared to CEF.
Cells. 2021. 10, 248
Discordance between the predicted vs. the actually recognized CD8+ T cell epitopes of HCMV pp65 antigen and aleatory epitope dominance
Frontiers in Immunology. 2021. 11: 618428
Functional T cell reactivity to melanocyte antigens is lost during development of malignant melanoma, but is restored with successful immunotherapy.
Cancers,13: 223 2021.
Aleatory epitope recognition prevails in human T cell responses?
Critical Reviews in Immunology 2020, 40: 225-235.
IL-21 in conjunction with anti-CD40 and IL-4 constitutes a potent polyclonal B cell stimulator for monitoring antigen-specific memory B cells.
Cells,2020, 9: 433.
Comprehensive evaluation of the expressed CD8+ T cell epitope space using high-throughput epitope mapping.
Frontiers in Immunology, 2019,10: 655.
Detection of Antigen-Specific T Cell Lineages and Effector Functions Based on Secretory Signature.
J Immunological Sci. 2019, 3, 14-20. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Cytotoxicity, Granzyme B, and Perforin ELISPOT - ELISPOT-related Reviews/Book Chapters -
Direct detection of T and B memory lymphocytes reveals HCMV exposure that serum antibodies fail to identify.
Cells. 2018, 19, 7. Transplantation Immunology - Infectious and Other Diseases -
B cells and B cell blasts withstand cryopreservation while retaining their functionality for producing antibody.
Cells. 2018, 7, 50 Fresh versus Cryopreserved PBMC -
High-throughput GLP-capable target cell visualization assay for measuring cell-mediated cytotoxicity.
Cells. 2018, 7, 35. Cytotoxicity, Granzyme B, and Perforin ELISPOT - Tumor Immunology -
Reagent Tracker Dyes permit quality control for verifying plating accuracy in ELISPOT tests.
Cells. 2018, 3, 7. Working with Peptides and Peptide Pools -
A positive control for detection of functional CD4 T cells in PBMC: The CPI pool.
Cells. 2017, 6, 47 Working with Peptides and Peptide Pools -
Delayed activation kinetics of Th2 and Th17 vs. Th1 Cells.
Cells. 6, 47 Measuring Th1, Th2, Th17, and Other Effector Lineages -
Multi-color FLUOROSPOT counting using the ImmunoSpot® Fluoro-X Suite.
Methods in Molecular Biology. 2018, 1808, 115-132. ELISPOT-related Reviews/Book Chapters -
Detecting all Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assay.
Methods in Molecular Biology. 2018, 1808, 85-94. ELISPOT-related Reviews/Book Chapters -
Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.
Methods in Molecular Biology. 2018, 1808, 73-84 ELISPOT-related Reviews/Book Chapters -
Four color ELISPOT assay for the identification of Effector T cell subpopulations.
Methods in Molecular Biology. 2018, 1808, 51-62 Measuring Th1, Th2, Th17, and Other Effector Lineages - ELISPOT-related Reviews/Book Chapters -
Multiplexing T- and B-cell FLUOROSPOT assays: Experimental validation of the multi-color ImmunoSpot® Software based on center of mass distance algorithm.
Methods in Molecular Biology, 3rd Edition (CH 9). 2018, 1808, 95-114. Cognate Cytokine Production by T Cells versus Bystander Cytokine Production -
Secondhand smoke impairs immune responses to respiratory infections.
J. Immunology. 2018, 15, 2927-2940. Tumor Immunology -
The enteric nervous system is a potenital autoimmune target in multiple sclerosis.
Acta Neuropathologica. 2017, 134, 281-295. Autoimmunity
The correlation between the virus- and brain antigen-specific B cell response in the blood of patients with multiple sclerosis.
Viruses. 2016, 8, 105. AutoimmunityAutoimmunity
How frequently are predicted peptides actually recognized by CD8 cells?
Cancer Immunology, Immunotherapy. 2016, 65, 847-855. Single-cell Resolution and Frequency - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Infectious and Other Diseases -
ELISPOT assays in 384-well format: Up to 30 data points with one million cells.
Cells. 2015, 4, 71-83. Single-cell Resolution and Frequency -
ELISPOTs produced by CD8 and CD4 cells follow Log Normal size distribution permitting objective counting.
Cells. 2015, 4, 56-70. Spot Size Distribution -
Characterization of the HCMV-Specific CD4 T cell responses that are associated with protective immunity.
Cells. 2015, 7, 4414-4437. Single-cell Resolution and Frequency - Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Cytokine Coexpression/Two-color ELISPOT Assays - Spot Size Distribution - Infectious and Other Diseases -
Normal distribution of CD8+ T cell-derived ELISPOT counts within replicates justifies the reliance on parametric statistics for identifying positive responses.
Cells. 2015, 4, 96-111. Single-cell Resolution and Frequency -
Serial measurements of apoptotic cell numbers provide better acceptance criterion for PBMC quality than a single measurement prior to the T cell assay.
Cells. 2015, 4, 40-55. Fresh versus Cryopreserved PBMC -
High reproducibility of ELISPOT counts from nine different laboratories.
Cells. 2015, 4, 21-39. Spot Size Distribution - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC -
Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood.
Clinical Immunology. 2014, 152, 20-24. Autoimmunity
Longitudinal T cell-derived IFN-γ/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis.
Journal of Immunological Methods. 2013, 398-399, 68-75. Cytokine Coexpression/Two-color ELISPOT Assays - Autoimmunity
An enhanced ELISPOT assay for sensitive detection of antigen-specific T cell responses to Borrelia burgdorferi.
Cells. 2013, 2, 607-620. Infectious and Other Diseases -
Chapter: When results of T cell immune monitoring match or do not match clinical outcomes of tumor vaccine trials — What more could and should we measure?
The Tumor Immunoenvironment. 2013, 725-740. Measuring Th1, Th2, Th17, and Other Effector Lineages - Tumor Immunology - ELISPOT-related Reviews/Book Chapters -
How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?
Viruses. 2012, 4, 2636-2649. Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Working with Peptides and Peptide Pools - Infectious and Other Diseases -
How ELISPOT morphology reflects on the productivity and kinetics of cells’ secretory activity.
Methods in Molecular Biology. 2012, 792, 125-143. ELISPOT-related Reviews/Book Chapters -
Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays.
Cells. 2012, 1, 313-324. Single-cell Resolution and Frequency - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC -
Optimal thawing of cryopreserved peripheral mononuclear cells for use in high-throughput human immune monitoring studies.
Cells. 2012, 1, 313-324. Fresh versus Cryopreserved PBMC -
Chapter: Statistical analysis of ELISPOT assays.
Methods in Molecular Biology. 2012, 792, 173-183. ELISPOT-related Reviews/Book Chapters -
Chapter: Unique strengths of ELISPOT for T cell diagnostics.
Methods in Molecular Biology. 2012. 792, 3-23. ELISPOT-related Reviews/Book Chapters -
T and B Cell Immunity is not Essential for the Induction of Proliferative Vitreoretinopathy.
Eye. 2012, 26, 872-881. Autoimmunity
The Magnitude of the Antigen-specific T Cell Response is Separated from the Severity of Spinal Cord Histopathology in Remitting-relapsing Experimental Autoimmune Encephalomyelitis.
Glia. 2012, 60, 794-805. Autoimmunity
Neuroantigen-Specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages.
Cells. 2012, 1, 576-596. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Autoimmunity
Cytomegalovirus (CMV)-specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease.
Cells. 2012, 1, 35-50. Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -
Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT.
Cells. 2012, 1, 127-140. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production -
Studies on the CNS histopathology of EAE and its correlation with clinical and immunological parameters.
Cells. 2012, 1, 576-596. Autoimmunity
Chapter: Objective, user-independent ELISPOT data analysis based on scientifically-validated principles.
Methods and Protocols, Handbook of ELISPOT. 2011, 792, 155-171. ELISPOT-related Reviews/Book Chapters -
The Immune Pathogenesis of Experimental Autoimmune Encephalomyelitis – Lessons Learned for Multiple Sclerosis?
Journal of Interferon & Cytokine Research. 2011. 31:907-16. Autoimmunity
Myelin-reactive antibodies mediate the pathology of MBP-PLP fusion protein MP4-induced EAE.
Clinical Immunology. 2011, 140, 54-62. Working with Peptides and Peptide Pools - Autoimmunity
Differential patterns of spinal cord pathology induced by MP4, MOG peptide 35-55, and PLP peptide 178-191 in C57BL/6 mice.
APMIS. 2011, 119, 336-346. Autoimmunity
Efficacy of a Tetravalent Chimeric Dengue Vaccine (DENVax) in Cynomolgus Macaques.
American Society of Tropical Medicine and Hygiene. 84(6), 2011. 978–987. Infectious and Other Diseases -
The Polyomavirus BK large T-antigen-derived peptide elicits an HLA-DR promiscuous and polyfunctional CD4+ T cell response.
Clinical Vaccine Immunology. 2011, 18, 815-824. Infectious and Other Diseases -
Recommendations from the iSBTc- SITC/FDA/NCI Workshop on Immunotherapy BioMarkers.
Clinical Cancer Research. 2011, 17, 3064-3076. Tumor Immunology -
The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood.
Clinical Immunology. 2010, 137, 422-432. ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Autoimmunity
Involvement of brain-derived neurotrophic factor (BDNF) in MP4-induced autoimmune encephalomyelitis.
Clinical Immunology. 2010, 137, 181-189. Autoimmunity
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer’s disease.
Neurobiology of Aging. 2010, 31, 1732-42. Infectious and Other Diseases -
Delineating the impact of neuroantigen vs genetic diversity on MP4-induced EAE of C57BL/6 and B6.129 mice.
APMIS. 2009, 117, 923-35. Autoimmunity
ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators.
Journal of Immunotoxicology. 2009, 6, 227-34. Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC -
Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function.
Journal of Virology. 2009, 83, 11175-11187. ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -
Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cells.
Journal of Immunology. 2009, 183, 5654-5661. Measuring Th1, Th2, Th17, and Other Effector Lineages - Vaccine and Adjuvants - Autoimmunity
Dissociated induction of cytotoxicity and DTH by CFA and CpG (41.8)
Journal of Immunotherapy. 2009, 32, 389. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytotoxicity, Granzyme B, and Perforin ELISPOT - Vaccine and Adjuvants -
Accessory cell dependent NK cell mediated PBMC IFN-gamma production is defective in HIV infection.
Clinical Immunology. 2009, 131, 288-297. ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -
Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells.
Cellular Immunology. 2009, 258, 131-137. Spot Size Distribution -
Perspective: research highlights at the Case Western Reserve University Center for AIDS Research. Learning from the past and looking toward the future.
AIDS Research and Human Retroviruses. 1996, 12, 1659.
Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.
Infectious and Other Diseases -Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.
Clinical Immunology. 2008, 129, 256-267. Autoimmunity
The TRAIL of helpless CD8+ T cells in HIV infection.
AIDS Research and Human Retroviruses. 2008, 24, 1-9. Measuring Th1, Th2, Th17, and Other Effector Lineages - Infectious and Other Diseases -
Antigen structure influences helper T-cell epitope dominance in the human immune re- sponse to HIV envelope glycoprotein gp120.
European Journal of Immunology. 2008, 38, 1231-1237. Infectious and Other Diseases -
Lack of Disease Specificity Limits the Usefulness of in vitro Costimulation in HIV- and HCV-Infected Patients.
Clinical and Developmental Immunology. 2008, 38, 1231-1237. Infectious and Other Diseases -
Chapter: Validating reference samples for comparison in a regulated ELISPOT assay.
Validation of Cell-based Assays in the GLP Setting. (CH 9) 2008. ELISPOT-related Reviews/Book Chapters -
Making the jump to high throughput microbial detection
Medlab. 2008, 3, 30-32. Autoimmunity
Allogeneic transplantation of multiple umbilical cord blood units in adults: role of pretransplant-mixed lymphocyte reaction to predict host-vs-graft rejection.
Leukemia. 2008, 22, 1786-90. Transplantation Immunology -
Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice BMC.
Musculoskeletal Disorders. 2006. 7:32. Transplantation Immunology -
The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types.
Clinical Immunology. 2007, 124, 267-276. Single-cell Resolution and Frequency -
MP4- and MOG:35-55-induced EAE in C57BL/6 mice differentially targets brain, spinal cord and cerebellum.
Journal of Neuroimmunology. 2007, 189, 31-40. Autoimmunity
Kinetics and Organ Distribution of IL-17-Producing CD4 Cells in Proteolipid Protein 139-151 Peptide-Induced Experimental Autoimmune Encephalomyelitis of SJL Mice.
Journal of Immunology. 2007, 178, 1372-1378. Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity
Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood.
Journal of Neuroimmunology. 2007, 187, 20-30. Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity
Granzyme-B production, but not cytokine signatures distinguish recently activated CD8 memory cells from resting memory cells.
Cell Immunology. 2007, 247, 36-48. Cytotoxicity, Granzyme B, and Perforin ELISPOT -
Dissociation of EAE protective effect and allergic side reactions in tolerization with neuroantigen.
Journal of Immunology. 2006, 178, 4749-4756. Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity
Dissociated production of perforin, granzyme B and IFN-γ by HIV-specific CD8+ cells in HIV infection.
AIDS Research and Human Retroviruses. 2007, 24, 62-71. Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -
HIV Coinfection Impairs CD28-Mediated Costimulation of Hepatitis C Virus-Specific CD8 Cells.
Journal of Infectious Diseases. 2006, 194, 391-400. Infectious and Other Diseases -
MBP-PLP fusion protein-induced EAE in C57BL/6 mice.
Journal of Neuroimmunology. 2006, 177, 99-111. Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity
Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors.
BMC Musculoskeletal Disorders. 2005, 36. Transplantation Immunology -
The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice.
Journal of Neuroimmunology. 2005, 170, 105-114. Single-cell Resolution and Frequency - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Autoimmunity
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
AIDS. 2005, 19, 1967-1974. Vaccine and Adjuvants - Infectious and Other Diseases -
Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.
Transplantation. 2005, 80, 1316-1322. Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -
IL-2 absorption affects IFN- gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.
Cell Immunology. 2005, 237, 28-36. Cytokine Coexpression/Two-color ELISPOT Assays -
CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes.
Cell Immunology. 2005, 235, 1-11. Autoimmunity
Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE.
Cell Immunology. 2005, 235, 29-38. Infectious and Other Diseases -
Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis?
Journal of Immunology. 2005, 174, 4598-4605. Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Autoimmunity
Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls.
Clinical Immunology. 2005, 115, 102-114. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Working with Peptides and Peptide Pools - Autoimmunity
Chapter: Image analysis and data management of ELISPOT assay results.
2005, 302, 117-131. Spot Size Distribution - ELISPOT-related Reviews/Book Chapters -
Results of an ELISPOT Proficiency Panel Conducted in 11 Laboratories Participating in International Human Immunodeficiency Virus Type 1 Vaccine trials.
AIDS Research & Human Retroviruses. 2005, 21, 68-81. Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -
Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects.
Journal of Autoimmunity. 2004, 23, 45-54. Working with Peptides and Peptide Pools - Autoimmunity
T cell immunity induced by live, necrotic, and apoptotic tumor cells.
Journal of Immunology. 2004, 173, 1012-1022. Tumor Immunology -
The third signal in T cell-mediated autoimmune disease?
Journal of Immunology. 2004, 173, 92-99. Autoimmunity
Selective impairments in dendritic cell-associated function distinguish hepatitis C virus and HIV infection.
Journal of Immunology. 2004, 172, 4907-4916. Infectious and Other Diseases -
Tc1 effector diversity shows dissociated expression of granzyme B and interferon- gamma in HIV infection.
AIDS. 2004, 18, 383-392. Working with Peptides and Peptide Pools - Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -
T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading.
J Clin Immunol. 2004, 24, 327-339. Working with Peptides and Peptide Pools - Autoimmunity
CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.
Journal of Immunological Methods. 2004, 285, 223-235. Working with Peptides and Peptide Pools - Vaccine and Adjuvants -
Deficient IFN-gamma expression in umbilical cord blood (UCB) T cells can be rescued by IFN-gamma-mediated increase in NFATc2 expression.
Journal of Clinical Immunology. 2003, 23, 485-497. Transplantation Immunology -
Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunity.
Journal of Immunology. 2003, 171, 3941-3946. Vaccine and Adjuvants - Tumor Immunology -
Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice.
Infection and Immununity. 2003, 71, 1820-1827 Measuring Th1, Th2, Th17, and Other Effector Lineages - Vaccine and Adjuvants - Infectious and Other Diseases -
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Journal of Immunological Methods. 2003, 279, 111-121. Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Measuring Th1, Th2, Th17, and Other Effector Lineages - Infectious and Other Diseases - Spot Size Distribution -
Enzyme linked immunosorbent spot (ELISPOT) assay for interferon-gamma independently predicts renal function in kidney transplant recipients.
American Journal of Transplantation. 2003, 3, 878-884. Transplantation Immunology -
T-cell epitope mapping using the ELISPOT approach.
Methods. 2003, 29, 260-269. Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Journal of Immunological Methods. 2003, 278, 79-93. Working with Peptides and Peptide Pools - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC -
On the same cell type GPI- anchored normal cellular prion and DAF protein exhibit different biological properties.
Biochemical and Biophysical Research Communications. 2003, 303, 446-451 Infectious and Other Diseases -
Autoreactive T cells promote post-traumatic healing in the central nervous system.
Journal of Neuroimmunology. 2003, 134, 25-34. Autoimmunity
Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool.
American Journal of Transplantation. 2002, 2, 857. Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC - ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Transplantation Immunology -
Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes.
Diabetes/Metabolism Research and Reviews. 2002, 18, 367-380. Autoimmunity
CD4+ T cells recognizing specific antigen deposited in glomeruli cause glomerulonephritis-like kidney injury.
Clinical Immunology. 2002, 104, 161-173. Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity
Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity.
Journal of Immunology. 2002, 168, 6099-6105. Working with Peptides and Peptide Pools - Vaccine and Adjuvants - Tumor Immunology -
Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself.
Clinical Immunology. 2009, 133, 45-51. Tumor Immunology -
Comprehensive determinant mapping of the hepatitis C-specific CD8 cell repertoire reveals unpredicted immune hierarchy.
Clinical Immunology. 2002, 103, 264-276. Determinant Mapping/High-throughput Screening - Infectious and Other Diseases -
HIV long-term non-progressors maintain brisk CD8 T cell responses to other viral antigens.
AIDS. 2002, 16, 1113-1118. Infectious and Other Diseases -
Indirect IL-4 pathway in type 1 immunity.
Journal of Immunology. 2002, 168, 545-553. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Cytokine Coexpression/Two-color ELISPOT Assays -
Production of transforming growth factor-beta 1 (TGF-β1) expression in human monocytes infected with Mycobacterium avium at a single cell level by ELISPOT assay.
Journal of Immunological Methods. 2002, 259, 25-32. ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -
Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund’s adjuvant by systemic immunization.
Journal of Infectious Diseases. 2001, 184, 308-314. Vaccine and Adjuvants - Infectious and Other Diseases -
Immunomodulatory effect of extracorporeal photopheresis after successful treatment of resistant renal allograft rejection.
Photodermatology, Photoimmunology & Photomedicine. 2001, 17, 79-82. Transplantation Immunology -
A T cell clone's avidity is a function of its activation state.
Journal of Immunology. 2001, 167, 1353-1361 Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Spot Size Distribution - Working with Peptides and Peptide Pools -
Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell-dependent, but disease susceptibility is defined by the non-T-cell compartment.
Clinical Immunology. 2001, 98, 85-94. Infectious and Other Diseases -
Pulmonary mononuclear cell responses to antigens of Mycobacterium tuberculosis in healthy household contacts of patients with active tuberculosis and healthy controls from the community.
Journal of Immunology. 2000, 165, 1479-1485. ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -
Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity.
Journal of Immunological Methods. 2000, 240, 143-155. Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -
Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen.
Journal of Immunology. 2000, 165, 12781284. Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Transplantation Immunology -
Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production.
Journal of Immunology. 2000, 165, 1641-1651. Working with Peptides and Peptide Pools - Autoimmunity
Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant.
Journal of Immunology. 2000, 164, 5771-5781. Cytokine Coexpression/Two-color ELISPOT Assays - Vaccine and Adjuvants - AutoimmunityTransplantation Immunology -
Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis.
Journal of Immunology. 2001, 166, 4757-4764. Autoimmunity Single-cell Resolution and Frequency -
Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients.
Journal of Immunology. 2000, 164, 3723-3732 Single-cell Resolution and Frequency - Measuring Th1, Th2, Th17, and Other Effector Lineages - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -
Selective peptidomimetic blockers of autoantigen presentation: a novel therapeutic approach to autoimmune disease.
Trends in Pharmacological Sciences. 2000, 21, 79-80. Autoimmunity
Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity.
Journal of Immunology. 2000, 164, 1862-1872. Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Spot Size Distribution -
Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.
Journal of Immunology. 1999, 163, 2267-2285. Transplantation Immunology -
Prevention of murine EAE by oral hydrolytic enzyme treatment.
Journal of Autoimmunity. 1999, 12, 191-198. Autoimmunity
Adjuvant-guided type- 1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response.
Journal of Immunology. 1999, 162, 3942-3949. Determinant Mapping/High-throughput Screening - Vaccine and Adjuvants - Measuring Th1, Th2, Th17, and Other Effector Lineages - Measurements of Functional T Cell Avidity -
Enhanced responses to Mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis.
Journal of Infectious Diseases. 1998, 178, 1434-1445. ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -
Heat shock proteins and experimental autoimmune encephalomyelitis. II: environmental infection and extra-neuraxial inflammation alter the course of chronic relapsing encephalomyelitis.
Journal of Neuroimmunology. 1998, 90, 149-161. Autoimmunity
The fate of T cells in the brain: veni, vidi, vici and veni, mori.
American Journal of Pathology. 1998, 153, 677-680. Autoimmunity
Shifting T-cell activation thresholds in autoimmunity and determinant spreading.
Immunological Review. 1998, 164, 53-61. Autoimmunity
Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure.
Transplantation. 1998, 66, 219. Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -
Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis.
Science. 1998, 281, 703-706. AutoimmunityInfectious and Other Diseases -
Endogenous myelin basic protein inactivates the high avidity T cell repertoire.
Journal of Experimental Medicine. 1998, 187, 2055-2063. Measurements of Functional T Cell Avidity - Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Autoimmunity
High-resolution characterization of cytokine-producing alloreactivity in naive and allograft-primed mice.
Transplantation. 1998, 65, 906-914. Transplantation Immunology -
CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity.
Journal of Experimental Medicine. 1997, 186, 1623-1631. Measuring Th1, Th2, Th17, and Other Effector Lineages - Working with Peptides and Peptide Pools - Vaccine and Adjuvants -
Induction of T helper 2 immunity to an immunodominant allopeptide.
Transplantation. 1997, 64, 292-296. Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -
Determinant spreading of T helper cell 2 (Th2) responses to pancreatic islet autoantigens.
Journal of Experimental Medicine. 1997, 186, 2039-2043. Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity
Modulating autoimmune responses to GAD inhibits disease progression and prolongs islet graft survival in diabetes-prone mice.
Nature Medicine. 1996, 2, 1348-1353. Autoimmunity
Immunomodulation by proteolytic enzymes
Nephrology Dialysis Transplantation. 1996, 11, 952-955. Transplantation Immunology -
Nasal administration of glutamate decarboxylase (GAD65) peptides induces Th2 responses and prevents murine insulin-dependent diabetes.
Journal of Experimental Medicine. 1996, 183, 1561-1567 Autoimmunity
Induction of TH1 and TH2 immunity in neonatal mice.
Science. 1996, 271, 1728-1730. Measuring Th1, Th2, Th17, and Other Effector Lineages - Working with Peptides and Peptide Pools - Vaccine and Adjuvants -
The human immune system in hu-PBL-SCID mice.
Immunology Today. 1995, 16, 529-533. Transplantation Immunology -
T cell cross-reactivity between coxsackievirus and glutamate decarboxylase is associated with a murine diabetes susceptibility allele.
Journal of Experimental Medicine. 1994, 180, 1979-1984. Autoimmunity
Anti-SCID mouse reactivity shapes the human CD4+ T cell repertoire in hu-PBL-SCID chimeras.
Journal of Experimental Medicine. 1994, 180,1817-1827. Transplantation Immunology -
Limited T cell response to donor MHC peptides during allograft rejection. Implications for selective immune therapy in transplantation.
Journal of Immunology. 1994, 153, 938-945. Transplantation Immunology -
The SCID mouse environment causes immunophenotypic changes in human immature T-cell lines.
International Journal of Cancer. 1994, 56, 546-551. Tumor Immunology -
Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes.
Nature. 1993, 366, 69-72. Autoimmunity
Dominance and crypticity of T cell antigenic determinants.
Annual Review of Immunology. 1993, 11, 729-766. Autoimmunity
Determinant spreading and the dynamics of the autoimmune T-cell repertoire.
Immunology Today. 1993, 14, 203-208. Autoimmunity
Human mature T cells that are anergic in vivo prevail in SCID mice reconstituted with human peripheral blood.
Journal of Experimental Medicine. 1992, 175, 503-516. Transplantation Immunology -
Limited B cell repertoire in severe combined immunodeficient mice engrafted with peripheral blood mononuclear cells derived from immunodeficient or normal humans.
The Journal of Clinical Investigation. 1991, 87, 658-665. Transplantation Immunology -
Graft-versus-host resistance induced by class II major histocompatibility complex-specific T cell clones.
Journal of Experimental Medicine. 1991, 173, 333-341. Transplantation Immunology -
Induction of graft versus host-associated immunodeficiency by CD4+ T cell clones.
Journal of Immunology. 1990, 145, 2092-2098. Transplantation Immunology
Natural T cell autoreactivity to melanoma antigens: clonally expanded melanoma-antigen specific CD8 + memory T cells can be detected in healthy humans.
Cancer Immunol. Immunotherapy 2019
Spreading of T-cell autoimmunity to cryptic determinants of an autoantigen.
Nature. 1992, 358, 155. Autoimmunity
Flexibility of the T cell repertoire. Self tolerance causes a shift of T cell receptor gene usage in response to insulin.
Journal of Experimental Medicine. 1990, 171, 1665-1681. Autoimmunity
Acute lethal graft-versus-host reaction induced by major histocompatibility complex class II-reactive T helper cell clones.
Journal of Experimental Medicine. 1990, 171, 1485-1496. Transplantation Immunology -
Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking.
European Journal of Immunology. 1989, 19, 1071-1077. Working with Peptides and Peptide Pools -
Why peptides? Their possible role in the evolution of MHC-restricted T-cell recognition.
Immunology Today. 1989, 10, 132-138 Working with Peptides and Peptide Pools -
In vivo competition between self peptides and foreign antigens in T-cell activation.
Nature. 1988, 334, 623-625. Working with Peptides and Peptide Pools -
Interaction of an immunodominant epitope with Ia molecules in T-cell activation.
Proceedings of the National Academy of Sciences. 1988, 85, 5181-5185. Working with Peptides and Peptide Pools
Quantitative HLA-class-II/factor VIII (FVIII) peptidomic variation in dendritic cells correlates with the immunogenic potential of therapeutic FVIII proteins in hemophilia A.
J Thromb Haemost 2020,18, 201-216.No Results Found