T Cell ELISPOT Testing

Let Our Contract Research Laboratory (CRO) Team Fast-Track Your T Cell ELISPOT Needs

As pioneers in the ELISPOT arena, the CRO Services team has more than a decade of proven expertise in the field of T cell ELISPOT.

Our CRO has developed and validated various customized T cell ELISPOT assays for pharmaceutical and biotechnology clients—large and small—spanning diverse antigenic systems, disease and treatment areas. We have successfully optimized and deployed high-throughput assays for key clinical trials (Phase I, II, and III) and testing for translational and preclinical studies.

Our CRO offers both, ready-to-go, kit-based T cell ELISPOT assay services and custom-designed assays to fit our clients’ specific project needs. Let us help you to get your project on the right footing. Reach out to our experienced immune monitoring team today!



t cell wells


Our T cell ELISPOT assays can utilize either fresh or cryopreserved functional PBMC

Optimized ELISPOT/FluoroSpot assays can reliably detect antigen-specific T cells even if they occur in frequencies of 0.0001% within PBMC (which is a single antigen specific analyte-secreting T cell within a million bystander cells).

These immune assays range from single color/analyte ELISPOT assays to novel, high-throughput multiplexed FluoroSpot assays, that can be tailored to fit your specific project needs. Beyond clinical studies, our contract laboratory services have developed T cell ELISPOT assays for other species including non-human primates (NHP), rat, murine, feline, and porcine systems, just to name a few.

Our T cell ELISPOT/ImmunoSpot® method allows for complete characterization of immunogenicity:

  • Testing T cell-mediated immunity for pre-clinical and clinical specimens
  • High-throughput assay testing capabilities
  • Epitope mapping with overlapping peptides
  • Measurement of CD4+ and CD8+ T cell responses
  • Performing multiple assays with the same sample material
  • Immunotherapeutics (e.g., oncology, autoimmunity)
  • Vaccine studies (e.g., infectious diseases, cancer vaccines)
  • Transplantation (e.g., allo, MLR)
  • Gene therapies (e.g., AAV-9)
  • Safety assessments (e.g., ADA)


Our highly trained immune monitoring experts can help coordinate multiple clinical sites and can walk clients through the complex aspects of T cell ELISPOT assay development, validation, deployment, and study logistics. Reach out to us to discuss your specific T cell ELISPOT project needs. To further explore our extensive T cell assay services and related products, please refer to our T cell ImmunoSpot® kits and our CRO brochure.


Reach out to us to discuss your specific T cell ELISPOT project needs.

To further explore our extensive T cell assay services and related products, please refer to our T cell ImmunoSpot® kits and our CRO brochure.



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