B Cell ELISPOT Testing
Let Our Contract Research Laboratory (CRO) Team Fast-Track Your B Cell ELISPOT Needs
As pioneers in the ELISPOT arena, the CRO service team has more than a decade of proven expertise in the field of B cell ELISPOT.
Our CRO has developed and validated various customized B cell ELISPOT assays for pharmaceutical and biotechnology clients—large and small—spanning diverse antigenic systems, disease and treatment areas. We have successfully optimized and deployed high-throughput assays toward sample testing in clinical trials (Phase I, II, and III) and translational and preclinical studies.
Our CRO offers both, ready-to-go, kit-based B cell ELISPOT assay services and custom-designed assays to fit our clients’ specific project needs.
Let us help you to get your project on the right footing. Reach out to our experienced immune monitoring team today!
Our B cell ELISPOT assays can reliably utilize either fresh or cryopreserved functional PBMC.
Optimized ELISPOT/FluoroSpot assays can reliably detect antigen-specific B cells even if they occur in frequencies of 0.0001% within PBMC (which is a single antigen specific analyte-secreting B cell within a million bystander cells).
These immune assays range from single color/analyte ELISPOT assays to novel and high-throughput multiplexed FluoroSpot assays that can be tailored to fit your specific project needs. Beyond clinical studies , our CRO has developed B cell ELISPOT assays for other species including non-human primates (NHP), rat, murine, feline and porcine systems, just to name a few.
We offer a wide range of single and multiplexed B cell ELISPOT/FluoroSpot assays to detect antigen-specific B cells, memory B cells, and to dissect antibody isotypes. Our highly trained immune monitoring experts can help coordinate multiple clinical sites and can walk you through the complex aspects of B cell ELISPOT assay development, validation, deployment and study logistics.
Our B-cell ELISPOT immunoassay services can accurately measure antigen-specific B cell populations:
- Antibody secreting cells (ASC)/plasma cells, which are a broad category of antigen-experienced, highly differentiated B cells that are generally considered to be short-lived.
- Memory B cells, which are known to be maintained for years or a lifetime and can be reactivated during repeat exposure to the same or closely related antigens.
While the precise ontogeny of these two populations is highly complex and is continually being refined by immunologists, these two broad categories provide a practical approach to assess aspects of B cell immunogenicity following antigenic exposure. Given their sensitivity, specificity, accuracy and high-throughput nature, ELISPOT assays have emerged as a key component in monitoring B cell immunogenicity following natural infection, autoimmunity, oncology, during vaccination and other immunotherapeutic interventions. -and to reach your milestones faster.
In addition, CRO services provide assays that can assess antibody isotypes (IgA, IgG, IgM, IgE) and IgG classes (IgG1/2/3/4) of responding B cells in optimized single or multiplexed ELISPOT/FluoroSpot assays. To further explore our extensive B cell assay services and related products, please refer to our B cell ImmunoSpot® kits and our CRO brochure.
Many such optimized assay services have helped our Pharma and Biotech Clients evaluate B cell immunogenicity in preclinical studies and clinical trials where ELISA could lead to inconclusive results.
