B Cell ELISPOT Testing

Let Our CRO Team Fast-Track Your B Cell ELISPOT

As pioneers in the ELISPOT arena, the Contract Research Laboratory Services team has more than a decade of proven expertise in the field of B cell ELISPOT.

Our CRO has developed and validated various customized B cell ELISPOT assays for pharmaceutical and biotechnology Clients—large and small—spanning diverse antigenic systems. We have successfully deployed such optimized, high-throughput assays toward sample testing in key clinical trials and translational and preclinical studies.

Let us help you get your project on the right footing. Reach out to our experienced immune monitoring team today.

Single, double, and multi-color B cell ELISPOT and FluoroSpot images

Our B cell ELISPOT assays can reliably utilize either fresh or cryopreserved PBMC

These immune assays range from single color ELISPOTs to novel and high-throughput multiplexed FluoroSpot assays that can be tailored to fit your specific project needs. Beyond clinical studies and the human system, our contract laboratory services has developed B cell ELISPOT assays for other species including non-human primates (NHP), rat, murine, and porcine systems, just to name a few.

We offer a wide range of single and multi-color B cell ELISPOT and FluoroSpot services to detect antigen-secreting B cells, memory B cells, and multiplexed assays to dissect antibody isotypes. Our CRO offers both ready-to-go, kit-based ELISPOT assay services and custom-designed assays to fit our Client’s specific project needs. Our highly trained immune monitoring experts can help coordinate multiple clinical sites and can walk you through the complex aspects of B cell ELISPOT assay development, validation, deployment, and study logistics. Reach out to us to discuss your specific B cell ELISPOT project needs.

Our B-cell ELISPOT immunoassay services can accurately measure antigen-specific B cell populations:

  • Antigen secreting cells (ASC)/plasma cells, which are a broad category of antigen-experienced, highly differentiated B cells that are generally considered to be short-lived.
  • Memory B cells, which are known to be maintained for years or a lifetime and can be reactivated during repeat exposure to the same or closely related antigens.

While the precise ontogeny of these two populations is highly complex and is continually being refined by immunologists, these two broad categories provide a practical approach to assess aspects of B cell immunogenicity following antigenic exposure. Given their specificity, accuracy and high-throughput nature, ELISPOT assays have emerged as a key component in monitoring B cell immunogenicity following natural infection, or, during vaccination and other immunotherapeutic interventions.

We offer optimized B-cell ELISPOT assays that can accurately measure the frequency of both ASC and memory B cell populations.

In addition, CRO services provide assays that can assess antibody isotypes (e.g., IgA, IgG) of responding B cells in optimized single or multiplexed ELISPOT/FluoroSpot assays. To further explore our extensive B cell assay services and related products, please refer to our B cell ImmunoSpot® kits and our CRO brochure.

As a key mission, the CRO specializes in developing and validating custom immune assays that are tailored to fit our Clients’ specific project needs and antigenic systems.

Many such optimized assay services have helped our Pharma and Biotech Clients evaluate B cell immunogenicity in preclinical studies and clinical trials. Whether your interests are related to oncology, vaccine development, or infectious disease, our experienced immune monitoring team can help you get your project on the right footing.

Our experienced immune monitoring team can help you reach your project milestones faster. For further information or a quote, please contact us today.

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