This optimized Fluorospot assay reliably detects human IgG1 and IgG4 secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.
This ImmunoSpot® kit contains low autofluorescence PVDF plates, diluents, capture antibody, detection reagents and a polyclonal B cell stimulator (Human B-Poly-S™) that promotes differentiation of B cells into antibody-secreting cells (ASC) capable of producing IgA, IgG or IgM.
