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Human IFN-γ/TNF-α/IL-2 Three-Color FluoroSpot

This optimized FluoroSpot assay reliably detects and measures human and some NHP IFN-γ, TNF-α, and IL-2 secretions by stimulated effector cells in frequencies as low as one in a million.

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate. Please contact us before placing your order if you are uncertain about your setup.

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Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit

Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.

Detection

Kit Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Volume discounts available, please inquire.

Components	Catalog #
IFN-γ/TNF-α/IL-2 Capture Kit	hT3001F
IFN-γ/TNF-α/IL-2 Capture Kit Precoated	hT3001Fp
IFN-γ Detection Kit	hT02
TNF-α Detection Kit	hT13
IL-2 Detection Kit	hT56

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Human IFN-γ/TNF-α/IL-2 Three-Color FluoroSpot

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Capture Kit

Detection

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