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Human IgG2/IgG4 Double-Color ELISPOT

This optimized ELISPOT assay reliably detects human IgG2 and IgG4 secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and polyclonal B cell stimulator (B-Poly-SE™) capable of eliciting IgE+ antibody-secreting cells (ASC)