This optimized FluoroSpot assay reliably detects and measures human IgG1, IgG2, and IgG3 secretions from either Antibody Secreting Cells (ASC) that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibodies, in frequencies as low as one in a million.
This ImmunoSpot® kit contains low autofluorescence PVDF plates, diluents, capture antibody, detection reagents and a polyclonal B cell stimulator (Human B-Poly-S™) that promotes differentiation of B cells into antibody-secreting cells (ASC) capable of producing IgA, IgG or IgM.
