S6 Flex M2

The S6 Flex M2 is a dedicated 4-color FluoroSpot reader, and so much more! It supports ELISPOT/FluoroSpot testing by enabling high-throughput PBMC viability counting, a rate limiting step for testing multiple PBMC samples in ELISPOT assays. The Flex is equipped with optics which, along with CTL’s dedicated LDA Cell Counting Suite permits live and dead cell counting in dozens of PBMC samples within minutes, including the documentation of the samples’ viability/fitness, and the calculation of resuspension volumes. The Flex is a multifunctional device that supports several assays which are standard in immune monitoring laboratories. Its live/dead cell counting ability, along with the TVA Cytotoxicity Suite, renders the Flex a high-throughput reader for cell-mediated cytotoxicity assays. Enabled by the BioSpot™ Software, this instrument permits automated reading of viral neutralization and infectivity assays in a wide range of well formats. The instrument comes with features supporting work in a regulated environment, and is high-throughput capable, handling up to 400 plates per run when equipped with our automated plate loader.

More Information



Well Formats

  • 24/48/96/384/1536 and Terasaki
  • Strip plate in 96 well format
  • (upgradable to rapid 6/Petri)

Advanced Components

  • Precision stage (0.24 mm stepping)
  • Digital area scan camera with image resolution of up to 10 Megapixels
  • Computer Interface: USB 3.0
  • Integrated Barcode Reader
  • Optical Interface: Fixed zoom with manual focus
  • Lighting Top: 96 LED Ring (rated for 100,000 operating hours)
  • Lighting Bottom: High illumination, uniform luminescent flat light panel
  • Lighting Fluorescence:
  • Long Life, Ultra-High Power BLUE LED
  • Long Life, Ultra-High Power GREEN LED
  • Long Life, Ultra-High Power VIOLET LED

Comes with Software

Data processing features

  • Dell Optiplex SFF computer
  • CPU: 8-core i7; Memory: 16GB RAM, Graphics: Intel HD
  • Storage: 500 GB SATA + 1TB GB SATA (System + Data); Optical Drive: Slimline DVD+/-RW
  • Ports: 1x Serial, 1x USB 3.1 Gen 2 Type-C, 4x USB 2.0 Type A, 5x USB 3.1 Type A, 1x Ethernet, 1x DP
  • Monitor: 24” Professional Widescreen
  • Keyboard/Mouse: US-Layout / Optical Mouse
  • Operating System: Windows 11 Pro with Microsoft Office 2021 Business


  • Imaging Unit (H x W x D); (Weight): 58.4 cm x 56 cm x 56 cm; (23” x 22” x 22”); 45.36 kg (100 lb)
  • Data Processing Unit (H x W x D); (Weight): 29 cm x 9 cm, 29 cm; (11.4” x 3.7” x 11.5”); 4.99 kg (11 lb)

Power requirements

  • Voltage: 120/230 V AC, 60/50 Hz

Optional components (sold separately)

  • Stacker (for 30 or 50 plates)
  • Robotic arm (for high-throughput plate loading – up to 400 plates)
  • IQ/OQ, ready for P/Q

Channel Excitation Filter Emission Filter Common Fluorophore
1 405 440 DAPI, Alexa Fluor® 405, Pacific Blue™, Po-Pro™, Sytox Blue™, ODense
2 480 520 FITC, Alexa Fluor® 488, Acridine Orange, sfGFP, GFP, Yo-Pro™, CFSC, Sytox™ Green
3 550 580 PE, Cy3, TRITC, CTL-Yellow, PE-Cy5.5, Sytox™ Orange
4 550 625 PI, dsRED, mRuby2
5 480 690 CTL-Red, PerCP, PE-Cy5.5
6 550 690 PE-Cy5, PE-Cy5.5
7 405 800 Qdot-800