This optimized FluoroSpot assay reliably detects mouse IgA, IgE, IgG, and IgM produced by antibody-secreting cells (ASC) that have either differentiated in vivo or were induced to differentiate into ASC following polyclonal stimulation in vitro.
This ImmunoSpot® kits contains low autofluorescence PVDF plates, diluents, capture antibody, detection reagents and a polyclonal B cell stimulator (Mouse B-Poly-S™) that promotes differentiation of B cells into antibody-secreting cells (ASC) capable of producing IgA, IgG or IgM.
