This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.

Reference Samples QC Set

The ePBMC® Reference Samples QC Set has been specifically designed for T cell and B cell assay development, qualification and validation.

This set is invaluable as internal controls for clinical trials, allowing researchers to use samples from donors with known reactivities to specific peptide antigens. Based on the validation results, the samples can be used to define cutoff values for acceptance of the assay. They are also ideal for testing the T and/or B cell assay proficiency of lab personnel, and for harmonizing T/B cell assays among different sites.

For T cell assays, PBMC are made available that display Th1 (IFN-γ or IL-2), Th2 (IL-4, or IL-5 or IL-13), Th17 (IL-17), or Th21 (IL-21) CD4+ T cell responses specific for select antigens included into the package. PBMC/peptide combinations are also available that induce granzyme B or perforin production in CD8+ T cells.

For B cell assays, the frequency of antibody-secreting cells (ASC) secreting immunoglobulin classes/subclasses has been established against antigen probes representing SARS-CoV-2, seasonal influenza, EBV and HCMV. Custom immune characterization is also available upon request.

Cat# CTL-QC1
≥10 million cells per vial
$180.00/vial

Volume discounts available, please inquire.

More Information

  

Details

Our Cryopreserved PBMC are ideal for the following applications:
  • ELISPOT/FluoroSpot Assays
  • Tetramers/Pentamers/Dextramers
  • Intracytoplasmic Cytokine Staining (ICS)
  • Cytokine ELISA Assays
  • Cytokine Bead Arrays (CBA)
  • Cytokine Protein Arrays (CPA)
  • Cytokine mRNA Determinations (RT-PCR)
  • ADCC
  • Transmigration Assays
  • Any applications that require live, functional PBMC