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Recent Publications by CTL

Recent Publications by CTL

CTL prides itself on our active, in-house ImmunoSpot-oriented research efforts that collectively aim to advance this technology's practical application for immune monitoring. Meet our 16 MD/PhD level scientists and learn what motivates them. Here are their latest contributions:

Noémi Becza, Lingling Yao, Greg A. Kirchenbaum, and Paul V. Lehmann

Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot Analysis of Antigen-Specific Memory B Cells
Preprint
Greg A. Kirchenbaum, Graham Pawelec, and Paul V. Lehmann

The Importance of Monitoring Antigen-Specific Memory B Cells, and How ImmunoSpot® Assays Are Suitable for This Task
Cells 2025, 14(3), 223
Paul V. Lehmann, Alexey Y. Karulin, Noémi Becza, Lingling Yao, Zhigang Liu, Jack Chepke, Andrea Maul-Pavicic, Carla Wolf, Sebastian Köppert, Alexis V. Valente, Anton V. Gorbachev, Magdalena Tary-Lehmann and Greg A. Kirchenbaum

Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot® assays
Journal of Immunological Methods, 2025, Volume 537, 113817
Paul V. Lehmann, Zhigang Liu, Noémi Becza, Alexis V. Valente, Junbo Wang, and Greg A. Kirchenbaum

Monitoring memory B cells by next generation ImmunoSpot® provides insights into humoral immunity that measurements of circulating antibodies do not reveal
Methods Mol Biol, 2024, 2768: 167-200.
Lingling Yao, Noémi Becza, Andrea Maul-Pavicic, Jack Chepke, Greg A. Kirchenbaum, and Paul V. Lehmann

Four-color ImmunoSpot® assays requiring only 1-3 mL of blood permit precise frequency measurements of antigen-specific B cells secreting immunoglobulins of all four classes and subclasses
Methods Mol Biol, 2024, 2768: 251-272.
Noémi Becza, Zhigang Liu, Jack Chepke, Xing-Huang Gao, Paul V. Lehmann, and Greg A. Kirchenbaum

Assessing the affinity spectrum of the antigen-specific B cell repertoire via ImmunoSpot®
Methods Mol Biol, 2024, 2768: 211-239
Alexey Y. Karulin, Melinda Katona, Zoltán Megyesi, Greg A. Kirchenbaum and Paul V. Lehmann

Artificial intelligence-based counting algorithm enables accurate and detailed analysis of the broad spectrum of spot morphologies observed in antigen-specific B cell ELISPOT and FluoroSpot assays
Methods Mol Biol, 2024, 2768: 59-85.
Alexander A. Lehmann, Diana R. Roen, Zoltan Megyesi, and Paul V. Lehmann

Reagent Tracker™ platform verifies and provides audit trails for the error-free implementation of T cell ImmunoSpot® assays
Methods Mol Biol, 2024, 2768: 105-114.
Paul V. Lehmann, Diana R. Roen, Alexander A. Lehmann.

Unbiassed, high-troughput identification of T cell epitopes by ELISPOT
Methods in Molecular Biology 2023, 2673: 69-88.
Carla Wolf, Sebastian Köppert, Noémi Becza, Stefanie Kuerten, Greg A. Kirchenbaum, Paul V. Lehmann

Antibody levels poorly reflect on the frequency of memory B cells generated following SARS-CoV-2, seasonal influenza, or EBV infection
Cells, 2022, 11:3662 Watch seminar presentation
Sebastian Köppert, Carla Wolf, Noémi Becza, Giuseppe A. Sautto, Fridolin Franke, Stefanie Kürten, Ted M. Ross, Paul V. Lehmann, and Greg A. Kirchenbaum.

Affinity tag coating enables reliable detection of antigen-specific B cells in ImmunoSpot Assays.
Cells, 2021,10:1843
Alexander A. Lehmann, Greg A Kirchenbaum, Ting Zhang, Pedro A Reche, Paul V Lehmann.

Deconvoluting the T cell response to SARS-CoV-2: specificity versus chance- and cognate cross-reactivity.
2021. Frontiers in Immunology. 2021, 12: 982
Alexander A. Lehmann, Pedro A. Reche, Ting Zhang, Maneewan Suwansaard, and Paul. V. Lehmann.

CERI, CEF-X, and CPI: largely improved positive controls for testing antigen-specific T cell function in PBMC compared to CEF.
Cells, 2021, 10, 248.
Alexander A. Lehmann, Ting Zhang, Pedro A Reche, Paul V Lehmann.

Discordance between the predicted vs. the actually recognized CD8+ T cell epitopes of HCMV pp65 antigen and aleatory epitope dominance.
Frontiers in Immunology, 2021, 11: 618428
Anna Przybyla, Alexander A. Lehmann, Ting Zhang, Jacek Mackiewicz, Łukasz Galus, Greg A. Kirchenbaum, Andrzej Mackiewicz, Paul V. Lehmann.

Functional T cell reactivity to melanocyte antigens is lost during development of malignant melanoma, but is restored with successful immunotherapy.
2021 Cancers,13: 223
Alexander Lehmann and Paul. V. Lehmann.

Aleatory epitope recognition prevails in human T cell responses?
Critical Reviews in Immunology, 2020, 40: 225-235.
Fridolin Franke, Greg A. Kirchenbaum, Stefanie Kuerten, and Paul V. Lehmann.

IL-21 in conjunction with anti-CD40 and IL-4 constitutes a potent polyclonal B cell stimulator for monitoring antigen-specific memory B cells.
Cells, 2020, 9, 433.

Search all of our 217 ELISPOT/FluoroSpot-related publications by topic.

See CV of our lead scientist, Prof. Paul V. Lehmann

Recent Publications by CTL

CTL prides itself on our active, in-house ImmunoSpot-oriented research efforts that collectively aim to advance this technology's practical application for immune monitoring. Meet our 16 MD/PhD level scientists and learn what motivates them. Here are their latest contributions:

Search all of our 217 ELISPOT/FluoroSpot-related publications by topic.

See CV of our lead scientist, Prof. Paul V. Lehmann