S6 Entry M2

The spot counting accuracy of the ImmunoSpot® Single-Color ELISPOT Enzymatic Software Suite is based on rigorous experimental validation. This software implements “a single click of a button” counting algorithm based on 25 years of leading scientific expertise in ELISPOT. In “expert” mode, this software enables scientists to modify any counting parameter and to perform high content analysis based on spot sizes and morphologies, for example, to establish cell productivity and affinity distributions of antigen-specific B cells. Built in user authentication, audit trail and data security support transparent ELISPOT counting in a regulated environment.
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Two color ELISPOT analysis can be performed using precipitating substrates that have distinct color spectra with visible/white light illumination (without involving fluorescence).

The ImmunoSpot® Double Color Enzymatic Software Suite permits precise counting of single-color spots for either analyte, as well as double color spots produced by cells that co-express both analytes (J. Immunol. 2000, 164:1862). CTL offers a wide selection of double color enzymatic ELISPOT kits enabling such dual color enzymatic ELISPOT analysis.

Immune monitoring efforts frequently involve measuring serum antibodies' efficacy in neutralizing viruses, bacteria or toxins. ImmunoSpot® readers fully support such high throughput measurements, including colony counting. Each type of colony or viral plaque has unique morphologies requiring specialized image analysis solutions. The BioSpot™ Software Suite has been established to meet this challenge. The BioSpot™ Software can distinguish and count independently objects/colonies of up to 12 different visible colors with white light illumination.

CFR Part 11 compliant QC and audit trails support transparent colony counting in a regulated environment.

CTL’s philosophy is to offer instruments that support several functions commonly performed in immune monitoring laboratories. Enabled by the BioSpot™ Software Suite, ImmunoSpot® Readers are rendered high-end colony counters.

To view the full spectrum of assays that can be analysed with the BioSpot™ Software, follow this link please.

Enabled by CTL’s ODense™ Software Suite, ImmunoSpot® Instruments are capable of reading ELISA results in addition to nucleic acid and protein quantification. The work flow is streamlined from planning the plate lay-out to automated generation of the graphs, and the statistical analysis of the results. CFR Part 11 compliant QC and audit trails support work in a regulated environment.

CTL’s philosophy is to offer instruments that support several functions commonly performed in immune monitoring laboratories, saving our customers the expense of having to purchase and maintain a dedicated instrument for each, all included in the cost of a single device.

High throughput analysis is streamlined by the SpotMap™ Software Suite. In the assay-planning phase, plate layouts are created, and barcodes are generated for each plate. SpotMap™ links the spot counts for groups of repetitive wells (e.g. triplicates) to the donor sample IDs and antigens present in those wells, and passes on this information for automated statistical analysis and subsequent export to any standard database. With SpotMap™ complex assays can be evaluated “at a click of a button”.

The Image Annotator™ facilitates image analysis-oriented exchange between scientists themselves, or scientists and CTL's expert support team. Regions of interst on a well image are marked and annotated. This information along with the raw image and the counting parameters are bundled together to be passed on for discussion or consultation.

Starting June 1st 2023, CTL is providing a barcoded ELISPOT reference plate with every Analyzer delivery. The ExpressQualify™ tool automatically scans and counts the reference plate to verify that the Analyzer is still performing within factory specifications. To meet formal regulatory requirements and documentation, please consult our full-version ImmunoQualify™ package.

The SpotStat® Software Suite was developed for the statistical analysis of ELISPOT/Fluorospot data in immune monitoring, vaccine testing, and other fields of immunological or medical research.  SpotStat® implements a number of powerful parametric and non-parametric statistical methods for easy, streamlined analysis of data generated by CTL’s ImmunoSpot® platform. Learn More

The Reagent Tracker™ Suite provides audit trails for the accurate implementation of ELISPOT/FlouroSpot assays, revealing pipetting errors made (https://doi.org/10.3390/cells7010003). Biologically neutral dyes are used to colour different antigens and the Reagent Tracker™ Software checks whether in each assigned well the right antigen/colour has indeed been plated (revealing qualitative pipetting errors), and whether the antigen pipetting volume was correct (revealing quantitative pipetting errors). The Reagent Tracker™ Suite generates automatic printable reports and is also ideally suited for proficiency testing of lab personnel, and for training of novices in the lab.

CTL has been committed to elevating ELISPOT/FluoroSpot assays so they can meet regulatory requirements for transparency and audit trails. The Reagent Tracker™ Suite is one of the important steps in that direction.