This optimized FluoroSpot assay reliably detects and measures human IFN-γ, TNF-α and IL-4 secretions by stimulated effector cells in frequencies as low as one in a million.
Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate. Please contact us before placing your order if you are uncertain about your setup.
ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!
Capture Kit
Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.
Detection Kits
Kit Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.
