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Human IFN-γ/TNF-α/IL-5 Three-Color FluoroSpot

This optimized FluoroSpot assay reliably detects and measures human and some NHP IFN-γ, TNF-α, and IL-5 secretions by stimulated effector cells in frequencies as low as one in a million.

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate. Please contact us before placing your order if you are uncertain about your setup.

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Details

CTL offers the ONLY COMPLETE kit that comes with everything you need – ALL FROM ONE SOURCE!

Capture kits

This ImmunoSpot® Capture kit includes diluents, serum-free assay medium and either capture antibodies for each cytokine and low autofluorescence PVDF-membrane plates or pre-coated low autofluorescence PVDF-membrane plates.

Detection kits

This ImmunoSpot® Detection kit contains detection antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Components	Catalog #
IFN-γ/TNF-α/IL-5 Capture Kit	hT3022F
IFN-γ/TNF-α/IL-5 Capture Kit Precoated	hT3022Fp
IFN-γ Detection Kit	hT02
TNF-α Detection Kit	hT13
IL-6 Detection Kit	hT41

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