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Human IFN-γ/TNF-α Double-Color ELISPOT

This optimized ELISPOT assay reliably detects and measures human and some NHP IFN-γ and TNF-α secretions by stimulated effector cells in frequencies as low as one in a million.

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Four-color T and B cell ELISPOT for simultaneous detection of analytes (266.46 KB)
96-well-counting (245.32 KB)
ImmunoSpot T Cell Kits Brochure (359.99 KB)
ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring — even in hands of ELISPOT-inexperienced investigators (312.75 KB)
High reproducibility of ELISPOT counts from nine different laboratories (534.74 KB)
ELISPOT assays in 384-well format: Up to 30 data points with one million cells (374.30 KB)
Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT assays (701.83 KB)
Optimal thawing of cryopreserved peripheral blood mononuclear cells for use in high-throughput human immune monitoring studies (237.77 KB)
Neuroantigen-specific CD4 cells expressing IFN-γ, IL-2 and IL-3 in a mutually exclusive manner prevail in EAE (572.39 KB)
Terms and Conditions (103.69 KB)

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