This optimized FluoroSpot assay reliably detects and measures human IgE and IgG4 secretions from either Antibody Secreting Cells (ASC) that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibodies, in frequencies as low as one in a million.
ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!
Includes Capture Antibodies, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.
Kit contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.