Human IgG1/IgG3/IgG4/IgM Four-Color FluoroSpot

This optimized Fluorospot assay reliably detects human IgG1, IgG3, IgG4 and IgM secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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Components Catalog #
Ig Capture Kit hB4F
IgG1 Detection Kit hB27
IgG3 Detection Kit hB41
IgG4 Detection Kit hB46
IgM Detection Kit hB05

Details

This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and polyclonal B cell stimulator (B-Poly-S™) capable of eliciting IgE+ antibody-secreting cells (ASC)