This optimized Fluorospot assay reliably detects mouse IgA,IgG1,IgG2a and IgM secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.
This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and polyclonal B cell stimulator (B-Poly-S™) capable of eliciting IgA, IgG1, IgG2a and IgM antibody-secreting cells (ASC).
Capture Kit
Includes low autofluorescence PVDF plates and all diluents.
Detection Kits
Kits contain unlabeled and/or fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.
