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Mouse IgG1/IgG2c Double-Color FluoroSpot

This optimized Fluorospot assay reliably detects mouse IgG1 and IgG2c secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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Details

This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and polyclonal B cell stimulator (B-Poly-S™) capable of eliciting IgG1 and IgG2c antibody-secreting cells (ASC).

Capture Kit

Includes low autofluorescence PVDF plates and all diluents.

Detection Kits

Kits contain unlabeled and/or fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Volume discounts available, please inquire.

Components	Catalog #
Ig Capture Kit	mB2F
IgG1 Detection Kit	mB26
IgG2c Detection Kit	mB43

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