This optimized Fluorospot assay reliably detects mouse IgG2a, IgG2b and IgG3 secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.
CTL offers the ONLY COMPLETE kit that comes with everything you need – ALL FROM ONE SOURCE!
Capture kits
This ImmunoSpot® Capture kit includes diluents, serum-free assay medium and either capture antibodies for each cytokine and low autofluorescence PVDF-membrane plates or pre-coated low autofluorescence PVDF-membrane plates.
Detection kits
This ImmunoSpot® Detection kit contains detection antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.
