This optimized ELISPOT assay reliably detects mouse IgG3 and IgM secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.
This ImmunoSpot® kit contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and a polyclonal B cell stimulator (Mouse B-Poly-S™) that promotes differentiation of B cells into antibody-secreting cells (ASC) capable of producing IgA, IgG or IgM.
