This optimized Fluorospot assay reliably detects mouse IgG1, IgG2b and IgM secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.
ImmunoSpot offers the ONLY COMPLETE kit which contains pre-coated PVDF plate, alkaline-phosphatase detection antibody, colorimetric substrate, diluents and serum free T-cell assay medium. Everything you need—all from ONE SOURCE!